单步溶胀聚合法制备单分散分子印迹聚合物微球:孔结构及其吸附与识别性能(英文)

Pore structure and binding character of uniform-sized molecularly imprinted polymer beads prepared by one-step swelling and polymerization

采用单步溶胀聚合法在水溶液中制得了单分散的S-萘普生分子印迹聚合物微球。利用扫描电镜及氮气吸附试验分析了微球的形态及孔结构,并考察了微球的吸附与识别性能。研究结果表明,通过单步溶胀聚合法制得的分子印迹聚合物微球具有较窄的粒径分布(ε<2.0%),氯仿及交联剂的用量对微球的表面形态、孔径大小及分布、比表面积等都有明显的影响。Scatchard分析表明分子印迹聚合物微球在识别S-萘普生分子过程中存在两类结合位点,高亲和力的结合位点的离解常数为Kd1=2.13mmol/L,Qmax1=36.46μmol/g,低亲和力的结合位点的离解常数为Kd2=31.55mmol/L,Qmax2=389.99μmol/g。

Molecularly imprinted polymeric microspheres （MIPMs） for S-naproxen were prepared in aqueous system by a one-step swelling and polymerization. The figurations and pore structures of the MIPMs were analyzed by scanning electron microscope and nitrogen adsorption, and the adsorption and recognition properties of the MIPMs were studied. Results of the studies indicated that molecularly imprinted polymer beads prepared by one-step swelling and polymerization had narrow size distribution （ε〈2.0% ）, and the dosage of chloroform and crosslinker influenced the configuration, pore diameter, pore diameter distribution and surface area of the MIPMs. Scatchard analysis shown the MIPMs recognizing S-naproxen with two classes of binding sites. The calculated dissociation constant Kd1 and apparent maximum number Qmax1 of binding sites with high affinity are 2.13mmol/L and 36.46gmol/g respectively, while Kd2 and Qmax2 Of binding sites with low affinity are 31.55mmol/L and 389.99μmol/g.