23个广西本地芒果品种的ISSR分析(英文)

Genetic Analysis of 23 Mango Cultivar Collection in Guangxi Province Revealed by ISSR

本研究对23个分别来自广西本地品种或广西选育的品种进行了SSR分析,并用15个芒果栽培品种印度(4个品种)、美国(3个品种)、缅甸(3个品种)、泰国(2个品种)及菲律宾、巴基斯坦和斯里兰卡(各1个品种)和2个芒果近源种冬芒(1个)和扁桃(1个)作为参照。从42个ISSR引物中筛选出9个多态性好的引物构建DNA指纹图谱来鉴别芒果的基因型。根据这9个引物获得的电泳图谱结果表明,本研究中所有供试品种能互相区分开来并表现出丰富的遗传多样性,说明ISSR是一种鉴定芒果品种(系)非常有效的方法。用74条多态性条带聚类分析作图发现所有供试的广西品种与秋芒、马切苏芒、象牙芒和爱文芒归为一个大类,而且广西本地芒果品种之间的亲缘关系较近。

Twenty-three mango cultivars （Mangifera indica Linn） collecting in Guangxi province were examined by ISSR markers and two species relatives M. himalis J.Y. Liang and M. persiciformis Wu ＆ Ming, other 15mango cultivars from India （4）, USA （3）, Burma （3）, Thailand （2）, Philippines （1）, Pakistan （1） and Sir Lanka （1）used as references. Of the 42 primers screened, 9 primers gave reproducible, polymorphic DNA amplification patterns, and were selected to construct a DNA fingerprinting map to distinguish the genotypes of mango. According to the banding patternsobtained with 9 selected primers, all cultivars tested in this study were distinguished from each other and showed ample genetic diversity, indicating that ISSR-PCR was an effective method for cultivar identification of mangoes. Based on 74 selected bands, all Guangxi mango cultivars tested were clustered into a big group with Neelum, Macheso, Aroemwnis, and Irwin by UPGMA analysis, indicating that Guangxi mango cultivars had a close relationship each other.