水稻与高粱属间远缘杂交花粉管行为观察

Pollen Tube Behaviour of Interspecies Hybridization between Rice and Sorghum Observation

水稻和高粱是禾本科的两个异属作物,因其遗传距离较远,杂交表现不亲和,结实率极低,难以获得种子。为初步了解水稻与高粱属间远缘杂交花粉管行为,探索该远缘杂交的发生过程,本实验以籼稻珍汕97A×珍汕97B和Ⅱ-32A×Ⅱ-32B两个组合为对照,对珍汕97A×晋江多枝高粱和Ⅱ-32A×晋江多枝高粱两个属间远缘杂交组合的花粉管行为作初步的观察与研究,结果表明:晋江多枝高粱花粉粒能象水稻花粉粒一样在水稻柱头上正常萌发,花粉管在花柱及子房中生长与伸长,有些能最终到达子房的基部;同时高梁花粉管生长也出现诸如不能进入花柱、胼胝质不规则堆积、花粉管末端钝化等一系列的异常表现,致使不少花粉管停滞生长。

Rice and Sorghum belongs to different genus. Distant hybridization between them becomes incompatible because of their further genetic distance. In order to detect the process of distant hybridization and the behavior of pollen tubes in the process, we conducted primary observation and research of the behavior of pollen tubes between two hybridization combinations of Zhenshan97A×Jinjiangduozhi sorghum and Ⅱ -32A×Jinjiangduozhi compared with that of Zhenshan97A×Zhenshan97B and Ⅱ -32A× Ⅱ -32B. The results showed that the pollen of Jinjiangduozhi sorghum can germinate on the rice stigma normally just as rice＇s pollen. The pollen tubes also can grow and elongate on the style and ovary, some can reach the base of the ovary eventually. Meanwhile, the pollen tubes of sorghum present a series of abnormal phenomenon, such as pollen tubes unable to enter into the style, callose depositing irregularly and the bottom of pollen tubes getting passivated, which brings about many pollen tubes stagnate.