血清和前列腺液中四种细胞因子水平与慢性非细菌性前列腺炎的关系

The relationship between four cytokines levels in serum and in prostatic fluid and chronic abacterial prostatitis

目的探讨血清和前列腺液中细胞因子与慢性非细菌性前列腺炎(CAP)的关系。方法用ELISA法对68例CAP患者血清和前列腺液进行IL-2、IL-4、IL-8、TNF-α检测,同时,以20例健康男性作正常对照,并对所取得的数据进行统计学处理。结果CAP患者血清和前列腺液中的IL-2、IL-8、TNF-α,以及患者血清IL-4均有不同程度的升高,与正常对照组相比差异显著(P<0.05或P<0.01),患者前列腺液中的IL-4水平与正常对照组比较无显著性差异(P>0.05)。结论IL-2、IL-8、TNF-α水平可能在CAP发生发展过程中起重要作用,对CAP辅助性诊断有较大应用价值;前列腺液中的IL-4水平与CAP症状间无相关关系(r=0.28,P>0.05),说明CAP的局部免疫反应可能不是以体液免疫为主,IL-4在其发病过程中不起主导作用;而患者血清中的IL-4水平与CAP间存在相关性(r=0.48,P<0.05),可能是细胞因子间网络样调节作用的结果。

Objective To explore the relationship between cytokines in serum and in prostatic fluid and chronic abacterial prostatitis （CAP）. Methods The levels of interleukin-2 （ IL-2）, interleukin-4 （ IL-4 ）, interleukin-8 （ IL-8 ）, tumor necrosis factor-α （TNF-α） were detected in 68 patients with CAP by enzyme linked immunosorbent assay（ ELISA）, and 20 healthy male volunteers as the control, and the data were statistically calculated. Results In patients with CAP, the serum and prostatic fluid levels of IL-2, IL-8, TNF-α and the serum level of IL-4 were significantly higher than those of the control （ P〈0. 05 or P〈0.01 ）. The level of IL-4 in prostatic fluid had not significant difference between the patients and the control（ P〉0. 05 ）. Conclusion The levels of IL-2, IL-8 and TNF-α play important roles in the occurrence and development of CAP, and they are effective for the auxiliary diagnosis of CAP. There is no correlation between the prostatic fluid level of IL-4 and symptoms of CAP（ r=0.28, P〉0. 05 ）, and it shows that IL-4 does not play a major role in the occurrence of CAP. There is correlation between the serum level of IL-4 and the occurrence of CAP（r=0. 48, P〈0.05 ）, which may be the result of cytokine network regulative function.