雌激素水平对三苯氧胺抑制乳腺癌细胞的体外研究

Study of Effects of Different Levels of Estradiol on Inhibitory of Tamoxifen on Human Mammary Cancer Cells(ER+) in Vitro

目的探讨雌激素水平对三苯氧胺(tamoxifen,TAM)抑制乳腺癌细胞MCF-7(ER阳性)生长的影响。方法应用不同浓度的雌二醇(estradiol,E2)和10μmol/L浓度的TAM作用于MCF-7细胞,应用四甲基偶氮唑蓝(MTT)比色法分析其对细胞生长的抑制作用。结果用1×10-12mol/L的E2开始刺激MCF-7细胞生长,到1×10-9mol/L时细胞生长达到平台期。10μmol/L的TAM可以明显抑制细胞生长,不同浓度的E2可以影响TAM对MCF-7细胞生长的抑制作用。当E2浓度达到1×10-8mol/L时,TAM对细胞生长的抑制作用最弱。结论E2是乳腺癌的危险因素,肿瘤细胞周围E2的浓度可以影响TAM的疗效。

Objective To study the effects of different levels estradiol on inhibitory of tamoxifen on human mammary cancer ceIIs（ER＋） in vitro. Methods Estrogen receptor （ER）-positive MCF-7 human breast cancer cell line was treated by the same level of tamoxifen and different levels of estradiol in vitro. Cell proliferation was evaluated by MTT assay. Results E2 at concentrations between 1× 10^-12 mol/L to 1×10^-7mol / L significantly stimulated the growth of MCF -7 . TAM （10 μmol/L） inhabited the growth of MCF-7 significantly. E2 at different levels may influence inhibitory of tamoxifen on MCF-7 cell lines. E2 （1×10^-8 mol/L） makes inhibitory of tamoxifen on MCF-7 cell lines valueless. Conclusion E2 is the risk factor of breast cancer, and the concentration around breast cancer cells may influence the effects of TAM.