碳离子局部照射对荷瘤小鼠存活时间和血常规的影响

Effects of local carbon ion irradiation on survival and peripheral blood leukocyte and platelet counts of tumor-bearing mice

目的观察碳离子局部照射对荷瘤小鼠存活时间和血常规的影响。方法将30只荷瘤小鼠随机均分为3组(0、1、24Gy组),用碳离子单次局部照射小鼠右后肢,分别在第0、7、14天计数外周血白细胞和血小板数,并记录小鼠生存时间。结果各组动物存活时间无显著性差异。照射后白细胞数都逐步升高,但与对照组(0Gy组)相比无显著性差异;除24Gy组第14天时血小板数显著升高外(P=0.032,F=4.062),照射后各组血小板数无明显变化。结论碳离子局部照射小鼠右后肢对动物存活天数和血常规无显著影响。

Objective To investigate the effects of local carbon ion irradiation on the length of survival and peripheral blood leukocyte and platelet counts of mice inoculated with pulmonary tumor cells. Methods Thirty tumor-bearing mice were randomly divided into control group （tumor-bearing but without carbon ion irradiation, n=10）, 12 Gy group （n=10） and 24 Gy group （n=10）. The right hind limbs of the mice, where the tumor cells were inoculated, were irradiated with carbon-ion beams at a single dose in 12 Gy and 24 Gy groups, and those of the control group received no irradiation. The peripheral blood leukocytes and platelets of the mice in all the 3 groups were counted immediately before and 7 and 14 days after irradiation, respectively, with the survival time of the mice recorded. Results There was no significant difference in the survival time of the mice between the 3 groups. The peripheral blood leukocyte counts in all groups increased after irradiation, no significant difference was noted between the two irradiation the groups and the control group. Irradiation at 24 Gy resulted in significant increase in peripheral blood platelet count on the 14th day （P=0.032, F=4.062）, but no such increment was observed in the other 2 groups or on day 7 in the 24 Gy group. Conclusion Local carbon ion irradiation may not produce significant effects on the length of survival and hemogram of the tumor-bearing mice.