肾上腺素对增生性瘢痕成纤维细胞TGF-β_1、bFGF及Ⅰ型前胶原表达的影响

The influence of adrenaline on the expression of TGF-β_1, bFGF and I procollagen for hypertrophic scar fibroblasts in vitro

目的实验研究肾上腺素对体外培养人皮肤成纤维细胞中Ⅰ型前胶原、TGF-β1及bFGF表达的影响。方法实验采用RT-PCR检测肾上腺素作用24h后bFGF、TGF-β1、人Ⅰ型前胶原的mRNA表达差异,并以ELISA检测bFGF、TGF-β1在肾上腺素作用24h后的蛋白表达差异。结果与对照组比较,肾上腺素上调bFGFmRNA及蛋白的表达和下调TGF-β1mRNA及蛋白的表达;但在增生性瘢痕成纤维细胞中,0.20μmol/L的肾上腺素可显著下调人Ⅰ型前胶原mRNA水平的表达,而在正常皮肤成纤维细胞中,0.05、0.1和0.2μmol/L的肾上腺素均可下调人Ⅰ型前胶原mRNA水平的表达,且其差异有统计学意义。结论在增生性瘢痕成纤维细胞生长融合后早期(24h),肾上腺素可促进成纤维细胞bFGF的mRNA和蛋白的合成,降低TGF-β1的mRNA和蛋白的生成,降低Ⅰ型前胶原mRNA的表达。

Objective To investigate the influence of adrenaline on the expresson of TGF-β1 , bFGF and Ⅰ procollagen for human normal and hypertrophic scar dermal fibroblasts cultured in vitro. Methods Human normal and hypertrophic scar dermal fibroblasts were propagated in a serum-free in vitro model with adrenaline for 24 hours. The human mRNA levels of bFGF, TGF-β1 and Ⅰ procollagen in fibroblasts were determined by RT- PCR. Levels of bFGF and TGF-β1 in the supernatants of fibroblasts cultured in vitro were determined by enzymelinked immunosorbent assay （EI,ISA）. Results In our study, adrenaline caused statistically significant increase in the peak levels of bFGF for normal and hypertrophic scar fibroblast cell lines （ P 〈 0.01 ）. It also caused statistically significant decrease in the level of TGF-β1 for normal and hypertrophic scar fibroblast cell lines. Modulation of normal fibroblasts with 0.05, 0. 10 and 0.20 μmol/L adrenaline resulted in a statictically significant （P 〈 0.01） decrease in the expression of Ⅰ procollagen mRNA. However, only 0.20 μmol/L adrenaline can decreased the mRNA expression of I procollagen in the hypertrophic scar fibroblasts. Conclusions We conclude from these results that adrenaline can increase the production of bFGF and decrease production of TGF-β1 and Ⅰ procollagen in human normal dermal and hypertrophic scar fibroblasts cultured in vitro.