柑桔皮水提取液急性毒性和致突变性

Studies on Acute Toxicity and Mutagenicity of Citrus Peel

背景与目的:对柚皮、橙皮水提取液进行毒理学安全性评价。材料与方法:采用昆明种小鼠经口急性毒性试验、Ames实验、小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸变试验进行毒理学检测。结果:柚皮、橙皮水提取液经口LD50>21.50g/kg,属无毒级。Ames实验,加S9和不加S9混合液的各剂量组回变菌落数与阴性对照差别无统计学意义,且无剂量反应关系。小鼠骨髓嗜多染红细胞微核试验、小鼠精子畸变试验与阴性对照组比较差别无统计学意义。结论:在实验条件下,柚皮、橙皮水提取液的经口LD50>21.50g/kg,属无毒级;未见有致突变作用。

BACKGROUND ＆ AIM： To study the acute toxicity and the mutagenicity of pummelo peel and orange peel. MATERIAL AND METHODS： The oral acute toxicity test in rats , Ames test, micronucleus test in bone marrow and sperm abnormality test were carried out. RESULTS： LD50 in rats of the pummelo peel and orange peel were more than 21.5 g/ kg. The micronucleus rates and sperm abnormality rates in all doses were not significantly different from the control group. No increase in the number of revertant colonies was found in the Ames test with and without S9 mixture. CONCLUSION： Acute toxicity and the mutagenicity of pummelo peel and orange peel were not observed in this study.