成年大鼠局灶性脑梗死后神经元前体细胞的迁移研究

Migration of neuronal precursors after focal cerebral ischemia in adult rats

目的探讨成年大鼠大脑中动脉栓塞缺血再灌注后梗死灶周神经元前体细胞的迁移特征。方法建立一侧大脑中动脉栓塞缺血再灌注模型,应用免疫组化及细胞记数检测缺血再灌注后1、7、14、21 d梗死灶周神经元前体细胞的变化。结果各个时相点的梗死灶周均可见神经元前体细胞;在缺血再灌注后第7天阳性细胞的数量达到高峰,随后逐渐下降;但在梗死区域的镜像区域未见到阳性细胞的表达。结论成年大鼠大脑中动脉栓塞缺血再灌注可激活神经元前体细胞的发生,并诱导神经元前体细胞朝着梗死区域迁移。

Objective To study the migration of neuronal precursors around the infarct area after focal cerebral ischemia. Methods Animal models of middle cerebral artery occlusion （MCAO） were established in 16 adult rats, and four of another 8 rats served as normal control and four underwent sham operation. Immunohistochemistry and cell count were applied to explore and contrast the migration of neuronal precursors for up to three weeks after a 2-hour episode of focal cerebral ischemia. Results The migration of neuronal precursors toward the infarct area could be found at various timepoints. The number of neuronal precursors started to increase in 1 d after reperfusion around the infarct area and reached the peak in 7 d, then declined till day 21. However, the neuronal precursors could not be examined in the corresponding non-ischemia area. Collclusiorl The neuronal precursors could migrate into the ischemic penumbra. It indicates that after cerebral ischemia, neuronal precursors are directed toward the sites of brain injury, where they might be in a position to participate in brain repair and functional recovery.