枸杞多糖对荷瘤小鼠免疫抑制因子VEGF、TGF-β1水平的影响

The effect on immunosuppressive factors VEGF,TGF-β1 of Lycium barbarum polysaccharide(LBP) in H_(22)-bearing mice

目的:观察枸杞多糖对荷瘤机体免疫逃逸的干预作用。方法:采用H22荷瘤小鼠,用枸杞多糖连续灌胃两周后观察胸腺、肿瘤重量,计算胸腺指数及肿瘤生长抑制率,ELISA双抗体夹心法检测各组血清中血管内皮细胞生长因子(VEGF)、转化生长因子β1(TGF-β1)水平。结果:枸杞多糖可明显抑制荷瘤小鼠肿瘤的生长,对小鼠的胸腺具有一定的保护作用,可下调血清中VEGF、TGF-β1水平。结论:枸杞多糖的抗肿瘤作用与抑制肿瘤机体VEGF、TGF-β1因子的分泌,干预机体的免疫逃逸状态有关。

AIM： To investigate the effection of Lycium barbarum polysaccharide（LBP） on the mechanisms of tumors escape immune recognition. Methods： LBP was given by oral to H22-bearing mice for two weeks, the therapeutic effect was determined by tumor weight, thymus index and the inhibition rate of tumor growth. The level of serum VEGF/TGF-β1 were measured by ELISA, Results： LBP can inhibit tumor cell proliferation, can protect thymus gland cells in a certain degree and can descent regulate the level of serum VEGF/TGF-β1. Conclusion： LBP had anti-tumor effect and is relevant by descent regulate the secretion of VEGF/TGF-β1 of tuomr cells.