黄芪保心汤抗心肌缺血与血管内皮保护作用研究

Protective effect of Astragalus Heart-Protecting Decoction(黄芪保心汤) on rats with acute myocardial ischemia

目的:探讨以益气活血化痰立法的黄芪保心汤防治心肌缺血的作用机理。方法:结扎冠状动脉造成大鼠急性心肌缺血损伤模型,以复方丹参滴丸作对照,观察两种药物对心电图、内皮素(ET)、一氧化氮(NO)、血栓素(TXB2)及6-酮-前列腺素F1α(6-keto-PGF1α)的影响。结果:心肌缺血大鼠存在明显的ET/NO、TXB2/6-keto-PGF1α平衡失调,与模型组相比,两种药物均能升高NO、6-keto-PGF1α水平,降低ET、TXB2水平,并能降低缺血心肌心电图ST段上移。结论:黄芪保心汤具有良好的抗心肌缺血作用,其作用机理可能与血管内皮保护效应密切相关。

Objective： To study the mechanism of Astragalus Heart-Protecting Decoction（AHPD） in prevention and treatment of acute myocardial ischemia（AMI） induced by ligation of coronary artery （LCA）. Method： 48 rats were randomly divided equally into four groups, group A was treated with Compound Danshen Dripping Pills（CDDP）, group B was treated with AHPD, group C and group D were seperately regarded as control group and sham-operated group. 2 weeks later, models of rats with AMI were made by LCA, After 3 hours, the elevation level of ST segment in ECG, the contents of ET, NO, TXB2 and 6-keto-PGF1α were examed. Result： After treatment, the level of ST segment and the contents of ET, TXB2 in AHPD group were lower than that in the control group（P〈0.05）, but the contents of SOD, 6-keto-PGF1α were significantly higher than that in AHPD group（P〈0.05）. As compared with CDDP group, there was no statistical difference （P〉0.05）. Conclusion： AHPD can act against myocardial ischemia effectively, one of the preventive and therapeutic mechanisms of which may be related with protecting the function of vascular endothelium,