摘要
为观察粉防己碱对人膀胱癌细胞株BIU 87的抑制作用并探讨其作用机制,采用四氮唑蓝法检测粉防己碱不同浓度对BIU 87细胞生长的抑制率,用流式细胞仪检测不同浓度粉防己碱作用后细胞的凋亡率,结果显示粉防己碱可有效抑制BIU 87细胞的生长,具有浓度依赖性特点,浓度为5～25mg/L的粉防已碱具有诱导人膀胱癌细胞凋亡的作用,且随着药物作用时间延长凋亡率逐渐增加,说明粉防己碱可有效抑制膀胱癌细胞生长,其作用机制与凋亡有关。
Objective： To observe the inhibitory effects of tetrandrine （Tet） on the BIU-87 cell in human bladder transitional cell eaneer（BTCC） and study its mechanism. Methods：The growth inhibition rates of BIU- 87 cell were investigated by MTT with various concentration Tet. Apoptosis rates were detected by flow eytometry after various time with various concentration Tet. Results： Tet effectively inhibited the growth of BIU-87 cell, which depended on concentration of the drug. Tet could induce the apoptosis of BIU-87 cells at its concentration being 5-25 mg. L^-1, and the apoptosis rates increased with the effect time of Tet. Conelusion：Tet could significantly inhibit the growth of bladder cancer cells. Inducing cell apoptosis might be one of its functional mechanism.
出处
《福建中医学院学报》
2005年第3期25-27,共3页
Journal of Fujian College of Traditional Chinese Medicine
关键词
粉防己碱
膀胱瘤
凋亡
细胞株
tetrandrine
bladder neoplasms
apoptosis
cell line
