双黄连注射液对小鼠血清组胺升高的影响

The Effect of Shuanghuanglian Injection on Elevating of Blood Serum Histamine in Mice

为探讨双黄连注射液导致变态反应的可能机制,采用ELISA和RIA方法测定健康昆明纯种小鼠腹腔注射双黄连注射液后的血清组胺和白细胞介素8(IL 8)的水平,结果实验组小鼠腹腔注射双黄连注射液后1h的血清组胺水平明显高于注射生理盐水的对照组(P<0 01),但IL 8水平与对照组比较无差异(P>0 05);注射后2h的组胺和IL 8水平与对照组比较无差异(P>0 05),说明双黄连注射液可导致实验小鼠的I型变态反应。

To explore allergic mechanism caused by Shuanghuanglian injection. To measure the concentrations of histamine and IL-8 in serum from health Kunming mouse after Shuanghuanglian injected with ELISA and RIA. The result showed there were significant difference between the concentration of histamine after 1 h in serum from the experimental mouse with and without administration of Shuang huanglian injection（P 〈 0.01）. It indicates allergic reaction of mice may be caused by receiving Shuanghuanglian injection.