卵巢上皮肿瘤组织Fas、Angs、E-cad的表达水平及意义分析

Expression of Fas, Angs, E-cad and the Significance Analysis in Ovarian Epithelial Tumor Tissues

目的检测Fas、促血管生成素-1(Ang-1)、促血管生成素-2(Ang-2)、E-钙粘蛋白(E-cad)在卵巢上皮肿瘤组织中的表达水平,藉此评价其与肿瘤发生发展及预后的关系。方法应用免疫组织化学S-P法和原位杂交技术,检查了21例卵巢上皮恶性肿瘤,18例良性肿瘤,8例正常卵巢组织中Fas,Ang-1,Ang-2及E-cad的表达。结果 Fas的表达随肿瘤病变程度的增高而逐步减弱,恶性肿瘤组织与正常卵巢及良性肿瘤组织间有显著差异(P<0．05)；E-cad免疫反应阳性强度在卵巢上皮癌组织中明显降低,与正常卵巢及良性肿瘤组织间有显著性差异(P<0．05)。原位杂交显示,Ang-1 mRNA在正常卵巢及肿瘤组织中都有表达,其表达水平没有显著差异(P>0．05)；Ang-2 mRNA在恶性肿瘤组织中表达明显上调(P<0．01)；E-cad mRNA在恶性肿瘤组织中表达明显下调(P<0.01)。结论 Fas的表达水平与卵巢上皮肿瘤的内在发展相关；由E-cad介导的细胞间黏附作用的减弱是卵巢癌发生、发展及转移的一个重要因素；Ang-2对于促进卵巢癌组织血管新生和形成可能具有重要促进作用。

Objective To examine the expression level of Fas, Ang-1, Ang-2, E-cad in human ovarian epithelial tumors and explore their relationship with tumorigenesis and prognosis. Methods Immunohistochemical S-P staining and in situ hybridization were used to determine the expression level of Fas, Ang-1, Ang-2, E-cad in benign, malignant tumors and normal ovarian tissues. Results （ 1 ） Immunohistochemical staining revealed that Fas expression decreased with malignancy degree of tumors and E-cad was weakly expressed in malignant tumor tissues as compared with benign tumors and normal ovarian tissues （ P 〈 0. 05 ） ; （2） In situ hybridization showed that Ang-1 mRNA expressed in both ovarian tumors and normal tissues and the difference in the expression level between them was not significant （ P 〉 0.05 ）. The expression of Ang-2 mRNA wasmore marked in malignant tumors than that in normal tissues and benign tumors （P 〈 0. 01 ）. The expression of E-cad mRNA was obviously down-regulated in malignant tumors （ P 〈 0.01 ）. Conclusion The expression level of Fas is correlated with internal development of ovarian epithelial tumors. The alteration of the intercellular adherence mediated by E-cad is an important factor in tumorigenesis and metastasis. Ang-2 may play an important role in promoting tumor angiogenesis in ovarian epithelial neoplasms.