骨力颗粒质量标准研究

Quality standard for Guli Granules

目的:制定骨力颗粒(淫洋藿、葛根、姜黄、牛膝、补骨脂等)的质量标准.方法:用TLC法鉴别葛根、姜黄、牛膝、补骨脂,以HPLC法测定淫羊藿苷的含量.选择C18柱,乙腈-水(28:72)为流动相,流速1.0 mL·min-1,检测波长270 nm.结果:TLC色谱中可明显鉴别葛根、姜黄、牛膝、补骨脂,淫羊藿苷线性范围17～85μg·mL～,平均回收率为(98.32±1.67)%.结论:定性、定量方法简便、可靠、准确、专属性强,可用于制剂的质量控制.

AIM ： To establish the method for quality control of Guli Granules （ Herba Epimedii, Radix Puerariae Lobatae, Rhizoma Curcumae Longae, Radix Achyranthis Bidentatae, Fructus Psoraleae, etc. ）. METHODS： Radix Puerariae, Rhizoma Curcumae Longae, Radix Achyranthis Bidentatae and Fructus Psoraleae in this medicine were identified by TLC. The icarrin content in this medicine was determined by HPLC. The acetonitril-water （28： 72） solution was used as the mobile phase, the column was C18, the flow rate was 1.0 mL · min^-1 The detection wavelength was at 270 nm. RESULTS： These herbs could be identified by TLC. For Icarrin, the linear range was from 17 to 85 μg · mL^-1, and the average recovery was 98.32% with RSD of 1.67%. CONCLUSION： The method is simple, reliable, accurate and can be applied to the quality control of the preparation.