摘要
目的:制定骨力颗粒(淫洋藿、葛根、姜黄、牛膝、补骨脂等)的质量标准.方法:用TLC法鉴别葛根、姜黄、牛膝、补骨脂,以HPLC法测定淫羊藿苷的含量.选择C18柱,乙腈-水(28:72)为流动相,流速1.0 mL·min-1,检测波长270 nm.结果:TLC色谱中可明显鉴别葛根、姜黄、牛膝、补骨脂,淫羊藿苷线性范围17~85μg·mL~,平均回收率为(98.32±1.67)%.结论:定性、定量方法简便、可靠、准确、专属性强,可用于制剂的质量控制.
AIM : To establish the method for quality control of Guli Granules ( Herba Epimedii, Radix Puerariae Lobatae, Rhizoma Curcumae Longae, Radix Achyranthis Bidentatae, Fructus Psoraleae, etc. ). METHODS: Radix Puerariae, Rhizoma Curcumae Longae, Radix Achyranthis Bidentatae and Fructus Psoraleae in this medicine were identified by TLC. The icarrin content in this medicine was determined by HPLC. The acetonitril-water (28: 72) solution was used as the mobile phase, the column was C18, the flow rate was 1.0 mL · min^-1 The detection wavelength was at 270 nm. RESULTS: These herbs could be identified by TLC. For Icarrin, the linear range was from 17 to 85 μg · mL^-1, and the average recovery was 98.32% with RSD of 1.67%. CONCLUSION: The method is simple, reliable, accurate and can be applied to the quality control of the preparation.
出处
《中成药》
CAS
CSCD
北大核心
2005年第11期1266-1269,共4页
Chinese Traditional Patent Medicine