灯盏细辛注射液HPLC图谱中两个主要峰的鉴定

Identification of two peaks in HPLC chromatogram of Erigeron Injection

目的:用HPLC图谱来研究灯盏细辛注射液的化学成分。方法:以HPLC分析为引导,采用硅胶柱层析,分离纯化主要色谱峰;结构鉴定采用UV、IR、MS、NMR及与文献有关数据比较。结果:从灯盏细辛注射液半成品分离得到二个化合物,根据理化常数和波谱分析鉴定为咖啡酸(Ⅰ)和绿原酸(Ⅱ)。结论:灯盏细辛注射液HPLC图谱的两个主要峰为Ⅰ和Ⅱ。

AIM ：To study the chemical constituents of Erigeron Injection by HPLC. METHODS： Under the guidance of HPLC analysis, two constituents were separated and purified by the aid of column chromatography with silica gel, and identified by UV, IR, MS, NMR respectively and documentary data. RESULTS： Two compounds from semi-finished products of Erigeron Injection were identified as caffeic acid （ Ⅰ ） and chlorogenic acid （ Ⅱ ）. CONCLUSION： Two main peaks in HPLC chromatogram of Erigeron Injection are Ⅰ and Ⅱ.