摘要
目的研究神经元凋亡在大鼠缺氧缺血性脑损伤(HIBD)中的地位及作用。并探讨选择性一氧化氮合酶抑制剂—7-硝基-吲唑(7-NI)对大鼠HIBD时神经元凋亡的影响。方法24h时用HE染色、TUNEL法、透射电镜观察和检测神经元凋亡并评估7-NI对神经元凋亡的影响。结果HIBD后24h在海马、皮质等处检测到神经元凋亡。7-NI使海马及皮质部位的神经元凋亡数显著降低。结论神经元凋亡参与了HIBD后神经元迟发性死亡。7NI有显著的抗HIBD后神经元凋亡的作用。
Objective To study the status and contribution of neuronal apoptosis after hypoxic-ischemic brain damage (HIBD) in rat. And to evaluate the effect of selective nitric oxide synthase inhibitor--7-NItroindazole (7-NI) on apoptosis of neurons after HIBD in rats. Methods HE staining, TUNEL staining and transmission electron microscopy were used to detected neuronal apoptosis 24 hours after HIBD. The effect of 7-NI on neuronal apoptosis was also studied. Results Neuronal apoptosis was detected in hippocampus and cortex at 24 h after HIBD. 7-NI can significantly reduced neuronal apoptosis after HIBD. Conclusions It is suggested that apoptosis account for important role of the delayed neuronal death after HIBD. It is selective NOS inhibitors--7-NI that shows its anti-apoptosis effect on HIBD 24 hours later.
出处
《科技通报》
2005年第6期689-692,696,共5页
Bulletin of Science and Technology
基金
浙江省自然科学基金资助项目(398428)
关键词
神经病学
凋亡
脑损伤
缺氧缺血
7-硝基-吲唑
neurology
apoptosis
brain damage
hypoxic-ischemic
7-NItroindazole(7-NI)