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肾上腺髓质素抑制醛固酮促大鼠主动脉外膜增殖效应 被引量：2

Adrenomedullin Inhibited the Effect of Aldosterone on Cell Proliferation in Rat Adventitia
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摘要 目的观察醛固酮(ALDO)对血管外膜肾上腺髓质素(ADM)产生和分泌的影响以及ADM mRNA表达水平,以探讨ADM和ALDO之间相互联系。方法用放射免疫法测定ALDO刺激后ADM产生和分泌水平,用3H-THR掺入反映血管外膜增殖,半定量的RT-PCR测定ADM基因表达。结果ALDO呈浓度依赖的刺激大鼠血管外膜ADM分泌和mRNA表达,ADM抑制ALDO诱导的外膜增殖。ALDO活化外膜成纤维细胞丝裂原活化蛋白激酶(MAPKs),明显增加其活性,该效应可被ADM抑制。结论ALDO促进血管外膜ADM的生成,而生成的ADM拮抗AL-DO诱导的血管外膜增殖。 Objective: To examine the effect of aldosterone on adrenomedullin(ADM) production and secretion and its mRNA expression in rat aortic adventitia to study the paracrine/autocrine interaction between endogenous ADM and aldosterone. Method: ADM produced and secreted from adventitia stimulated by aldosterone was detected by radioimmunoassay.Proliferation in adventitia cells was evaluated by the level of 3H-thymine incorporation.And prepro ADM gene expression was measured by semi-quantitative reverse transcriptase polymerase chain reaction. Results: Adventitial ADM secretion and mRNA expression stimulated by aldosterone were concentration-dependent and it was an inhibitive effect of ADM on aldosterone-induced proliferation. ADM inhibited the activity of mitogen activated protein kinases(MAPKs) stimulated by aldosterone.Conclusion: Aldosterone stimulates adventitia to produce and secrete ADM, which in turn antagonizes the aldosterone-induced proliferation in adventitia.
作者 吴胜英 蒋维 潘春水 齐永芬 汪雄 唐朝枢
机构地区 郧阳医学院病理生理学教研室 北京大学医学部生理与病理生理系
出处 《微循环学杂志》 2005年第4期1-5,共5页 Chinese Journal of Microcirculation
基金 国家重大基础研究发展规划项目(973)(G2000056905) 湖北省教育厅重点项目(2003A003)
关键词 肾上腺髓质素 醛固酮系统 增殖效应 动脉外膜 心血管组织 CYP11B2 大鼠 System 血管紧张素 心血管功能 Adrenomedullin(ADM) Aldosterone(ALDO),Adventitia,Proliferation
分类号 R544.1 [医药卫生—心血管疾病] R174.1 [医药卫生—妇幼卫生保健]
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参考文献13

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同被引文献16

  • 1赵林双,丁东平,向光大,戚本玲,柯琴梅,乐岭,侯洁,曹红艳.血管紧张素Ⅱ1型受体拮抗剂与醛固酮受体拮抗剂对逆转高血压伴左室扩大患者心肌重塑的作用[J].微循环学杂志,2005,15(1):49-51. 被引量：5
  • 2胡文兰,王晋明,胡钢英,梁远红,王芳,孙琦.缬沙坦和螺内酯抑制自发性高血压大鼠心肌纤维化的对比研究[J].中华老年医学杂志,2004,23(12):875-878. 被引量：6
  • 3邢玉,高丽,万国华,张建新,李兰芳.螺内酯对高血压大鼠主动脉重塑及对血浆肾上腺髓质素的影响[J].河北医药,2005,27(8):572-574. 被引量：1
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微循环学杂志
2005年 第4期

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