过继免疫治疗对手术后肺癌患者外周血免疫细胞表型影响的研究

Effects of CIK on the Lymphocytes Immunophenotype in Patients with Lung Cancer

目的:探讨C IK过继免疫治疗对手术后肺癌患者免疫功能的影响。方法:采用流式细胞术检测40例肺癌患者外周血T细胞、B细胞和NK细胞表型。结果:肺癌患者CD3+、CD4+、CD4/CD8比值明显低于对照组并呈显著性差异,而CD8+细胞高于对照组(P<0.01)。经C IK治疗后CD3+、CD4+、CD3-/CD16+56+细胞和CD4/CD8比值都升高,其中CD4+、CD3-/CD16+56+细胞与治疗前比较呈显著性差异(P<0.05),而CD8+细胞与治疗前比较降低(P<0.05);CD19在治疗后稍有增高趋势,但无显著性差异。结论:C IK过继免疫治疗对肺癌患者免疫功能的恢复具有促进作用。

Objective： To investigate the immunophenotype of patients with lung cancer treated with CIK. Methods： The T cell and B cell subsets and NK cells in peripheral blood from patients with lung cancer （ n = 20） were detected with flow cytometry. Results： The CD3 ^＋, CD4^ ＋ and CD4/CD8from pre - operation patients with breast cancer were significantly lower than that of the control （ P 〈 0. 01 ）. Compared with the control group, the CD8^ ＋ cells from patients with lung cancer were increased （P 〈0, 01 ）. The CD3^ ＋ , CD4 ^＋, CD3^ -/CD16 ＋ 56 ＋ cells and CD4/CD8 from post -operation patients treated with CIK were increased. Compared with pre- operation, the differences of CD4^ ＋, CD3^ -/CD16 ＋ 56 ＋ ceils were significant （ P 〈 0.05） and the CD8 ＋ ceils were decreased （ P 〈 0.05 ） , Conclusion： The immunological function of patients with lung cancer is decreased and con be enhanced by CIK adoptive immunotherapy.