马哈利樱桃砧木组培快繁技术研究

Study on rapid propagation technology of Prunus mahaleb

以马哈利樱桃砧木的嫩梢茎尖为外植体进行离体培养,筛选出的起始培养基为MS+6-BA 0．2- 0．5 mg／L;最佳增殖培养基为MS+6-BA 2．0 mg／L+IAA 0．5 mg／L+GA3 0．5 mg／L,此时增殖倍数达7．06,平均 苗高2．79 cm;最佳生根培养基为1／2 MS+IAA 2．0 mg／L或1／2 MS+IBA 0．6 mg／L,暗培养7 d后移至温室或 大棚,在自然光下生根炼苗,生根率达85％以上,单株平均根数3-6条,根系白嫩,组培苗生长整齐健壮;以营养土 为基质,用50 mg／L生根粉泥浆溶液处理生根组培苗的移栽成活率达87．0％以上。该技术体系适宜工厂化生产的 要求。

Vegetative axillary shoots were excised from field trees of Prunus mahaleb,then surfacesterilized and cultured on MS medium with 0. 2-0.5 mg/L 6-BA. The results showed that optimal medium for shoot proliferation were MS medium with 2.0 mg/L 6-BA and 0. 5 mg/L IAA and 0. 5 mg/L GA3. On this medium, the average propagation multiple was 7.06 and average height of plantlets was 2. 79 cm. Roots were induced on MS medium containing half-strength macroelements and 2. 0 mg/L IAA or 0. 6 mg/L IBA. After being cultured 7 days in the dark,axenic plantlets were transplanted to greenhouse for rooting and acclimation in the normal light ,more than 85 % plantlets were induced strong roots,the average number of roots each strain were 3-6. The plantlets dealt in slurry with 50 mg/L ABT1 were transplanted into nutrition soil and survival rate was more than 87.0 %. This propagation way can satisfy the need of factory production.