低次烟叶蛋白质提取工艺研究

Study on protein extraction technology from discarded tobacco leaf

以低次烟叶为材料,研究了烟叶蛋白质最佳提取工艺。采用L9(34)正交试验设计研究了烟叶磨浆 工艺。结果表明,磨浆工艺的最佳工艺参数为固液比(烟叶:水)1:17,提取温度60℃,提取液pH 8．0,磨浆2次。 采用响应曲面分析法研究了烟叶蛋白碱溶工艺,得到其最佳工艺参数为温度60℃,pH 8．0,时间60 min,搅拌条件 下碱溶3次。烟叶蛋白酸沉操作中,在pH 3．0,4℃下静置8 h效果最佳,最终烟叶蛋白提取率为76．62％。

The optimum extracting conditions of protein in discarded tobacco leaf was studied. The results showed that the optimum technology conditions for tobacco leaf grinding were as follows ：the ratio of 1 （tobacco leaf） ; 17（water）,temperature 60 C ,pH 8.0 and grinding twice with orthogonal experiment design methods L9（34）. The optimum extracting conditions of protein were 60 C ,pH 8.0,60 min and extracting three times with agitation by Response Surface Methodology. And the optimum conditions of depositing protein with acid were pH 3.0,4 C for 8 h. The extraction rate reached 76.62% under the optimal conditions.