皱马鞍菌液体培养过程中胞外酶及还原糖的动态变化

Changes of extra-cellular enzyme activity and reducing sugar consistency during the submerged culture of Helvella crispa

对皱马鞍菌(Helvella crispa)液体培养过程中不同胞外酶活性和还原糖含量的动态变化进行了研 究。结果表明,在28 ℃、110 r,／min恒温振荡培养条件下,皱马鞍菌菌丝体生物量在第13天达峰值(9．40 g／L);发酵 液pH值逐渐上升,从第9天(8．24)后基本保持平稳;胞外蛋白含量于第6天达峰值(8．30 mg／mL);胞外还原糖 含量急剧上升,至第3天达峰值(20．43 mg／mL),之后迅速下降,到第8天降为1．38 mg／mL,此后一直维持在1 mg／mL水平。皱马鞍菌对碳水化合物的利用顺序为淀粉、纤维素、木质素。淀粉酶的活性高峰出现在第3天,并一 直保持在较高水平;CMC酶6 d后急剧分泌,第7天达峰值,之后又急速下降;愈创木酚氧化酶和多酚氧化酶活性 高峰出现最晚,均在第14天。酸性蔗糖转化酶活性在培养期间始终很低,而中性蔗糖转化酶活性呈阶梯上升趋势。

Dynamic variation of extra-cellular enzyme activity and reducing sugar concentration during the submerged culture of Helvella crispa were investigated. The results showed that the maximum of hyphostroma was 9.40 g/L,and appeared on 13th day,pH of the fluid medium went up gradually and kept smooth after the 9th day. The maximum of extra-cellular protein consistency was 8. 30 mg/mL and appeared on the 6th day. During the submerged culture,reducing sugar concentration increased rapidly and come to the maximum （20.43 mg/mL） on the 3th day,and later the concentration decreased gradually. Amylum were decomposed earliest by Helvella crispa,next were cellulose and lignin. The peak of amylase activity appeared on the 3th day and kept the peak from beginning to end. The peak of CMCase （CMC.carboxymethyl cellulose） activity appeared on the 7th day while enzyme production of guaiacol oxidase and polyphenol oxidase reached the peak on the 14th day. The activity of acid invertase was very low and activity of neutral invertase increased steadily during submerged culture.