康氏木霉固态发酵生产纤维素酶条件的研究

Studies on the conditions of cellulse produced by Trichderma koningii with solid state fermentation

以麸皮和玉米芯为主要原料,采用康氏木霉(Trichoderma koningn)固态发酵生产纤维素酶,并对 影响纤维素酶活性的碳源和氮源配比、外加氮源、培养基初始pH值、培养时间和表面活性剂等因素进行了研究。结 果表明,培养基碳源和氮源配比以麸皮:玉米芯为3:2时最适合;外加氮源以质量分数0．8％硝酸铵产纤维素酶 活性最高;培养基初始pH值为3．0时,纤维素酶活力最高,是对照的1．37倍;表面活性剂以质量分数0．50％“奇 强”洗洁精对提高纤维素酶活性的作用最显著,较对照提高12．4％;培养时间为72 h时,纤维素酶活性最高。

Using wheat bran and corncob as main substances,cellulase is produced by Trichoderma koningii with solid state fermentation. The effect on cellulase activity is studied of proportioning of carbon and nitrogen source ,added nitrogen source,initial pH of medium,culture time and surfactant. The results indicated ：the proportioning of wheat bran and corncob is most suitable at 3 ： 2. The activities of enzyme is the highest when the mass concentration of added ammonium nitrate is 0.8%. When initial pH of medium is 3.0,and the activitiy of cellulase is the highest, 1. 37 times as much as that of the control. With washingup liquid as surfactant,the effect is the best,the activity is higher than control by 12.4% when its volume concentration is 0. 5%. When the culture time is 72 h,the activity of enzyme reaches the highest.