中国湖北地区汉坦病毒M片断基因变异的研究

Genetic Diversity of M Segment of Hantaviruses in Hubei Province,China

目的:了解中国湖北地区汉坦病毒的遗传学特征.方法:采集2000～2001年肾综合征出血热(HFRS)患者的血液标本,通过逆转录聚合酶链反应(RT-PCR)扩增汉坦病毒M片断基因.22例IgM阳性患者中,从 5例患者的血凝块中扩增得到特异性PCR产物.结果:对550bp和403bp扩增产物进行的系统进化分析揭示汉坦病毒至少存在3个不同分支.W613株和W1141株与原型株HTN76-118,CUMC-B11(韩国),cl-l和cl-2(日本)同一分支;W1219株和H8205同一分支;另外两株与A9和HV114同一分支.结论:首次发现湖北地区人类汉坦病毒株与韩国和日本的HTN型具有高度序列同源性.

Objective： To study the biology and molecular epidemiology of hantaviruses in Hubei province, China. Methods： The blood samples were collected from HFRS patients between 2000 2001 ,and the M segment genome of hantavirus was amplified by RT-PCR. Specific PCR product was obtained from blood clot of 5 of the 22 IgM-positive patients. Results： Phylogenetic analysis of the 550- and 403-base pair amplicons revealed that there were at least 3 genetically distinct hantavirus lineages. W613 and Wl141 strains clustered with the prototype strain Hantaan76-118, CUMC-B11 （Korea）, cl-land cl-2（Japan） ; Wl 219 strain, formed a lineage with H8205 ; 2 additional strains fell to the lineage of A9 and HVll4. Conclusion： This is the first report that human hantavirus strains（W613 and Wll41）from Hubei Province are found to share high sequence homology with Korean and Japanese THN viruses. This result shows that a highly divergent group of hantaviruses exist in this geographic region.