维生素A酸对小鼠囊胚Fas蛋白表达的影响

Effect of Retinoic Acid on the Expression of Fas Proteins in Mouse Blastocysts

目的:探讨全反式维生素A酸(ATRA)对体外培养小鼠囊胚Fas蛋白表达的作用,并了解其对着床前期胚胎发育的作用。方法:获取妊娠3.5 d小鼠囊胚,分别培养在含0、1μmol.L-1和10μmol.L-1的ATRA的M199培养基中24 h,用免疫组织化学SP法,分别检测不同剂量组ATRA对小鼠囊胚Fas蛋白表达的状况。结果:ATRA可增强小鼠囊胚Fas蛋白表达,表达强度呈现剂量依赖型。结论:ATRA对小鼠胚胎的发育具有细胞毒性作用,Fas在小鼠胚胎发育的调节中发挥了重要的作用。

Objective： To study the effect of all-trans retinoic acid （ATRA） on the expression of Fas protein in mouse blastocysts in vitro and its possible roles in preimpiantation mouse embryo development. Methods： Mouse 3.5 d blastocysts were grown in doses of 0, 1 and 10μmol · L^-1 alltrans retinoic acid for 24 h and observed for their expression of Fas proteins by immunohistochemical SP staining. Results： The expression of Fas protein affected by ATRT in mouse blastocysts was dose-dependent. Conclusion： This study examined the cytotoxic effect of ATRA on mouse embryos. Fas may play critical regulating roles in mouse embryo development.