摘要
目的:观察重度烧伤后肺组织几种炎症相关基因表达变化,探讨其与肺损害的关系.方法:采用大鼠40%体表面积,深Ⅱ～Ⅲ度烫伤模型,于伤后0(正常组),3,6,12,48,72h,用逆转录聚合酶链反应(RT-PCR)方法检测肺组织肿瘤坏死因子-α(TNF-α),白介素-6(IL-6),白介素-10(IL-10).结果:烫伤后3 h,TNF-α表达显著上调(P<0.01),此后一直维持高表达状态,于伤后48 h降至正常;IL-6于伤后6 h表达明显升高(P<0.01),伤后48h降至正常水平;IL-10于伤后下调并维持低水平.结论:炎症相关基因TNF-α、IL-6、IL-10参与了烧伤后肺组织局部失控性炎症反应,TNF-α、IL-6上调及IL-10表达下调可能是烧伤后早期肺组织损害的重要原因之一.
Objective： To observe expression of genes ter severe burn. Methods： After 40 percent of expression of tumor necrosis factor-α（TNF-α） related to inflammatory response in lung of rats afbody surface area （TBSA） of rat was burned gene , interleukin-6 （IL-6）, interleukin-10 （IL-10） in lungs was detected with reverse transcription polymerase chain reaction. （RT-PCR）. Results： TNF-α mRNA was increased dramatically at lhour after burn and maintained at high level up to 24 hours later （P〈0.01）. IL-6 gene expression was upregulated at 3 hours after burn （P〈0.01） and declined to normal level 12hours later. IL-10 gene was downregulated after burn and maintained low level down to 24 hours. Conclusion： Over expression of TNFα, and IL-6 and low expression of IL-10 mRNA in lungs of rats may be partial cause of local lung uncontrolled inflammatory response and then partially contributed to lung impairment after burn.
出处
《武汉大学学报（医学版）》
CAS
2005年第6期718-721,共4页
Medical Journal of Wuhan University
关键词
烧伤
肿瘤坏死因子-Α
白介素-6
白介素-10
Burn
Tumor Necrosis Factor-α（TNF-α）
Interleukin-6 （IL-6）
Interleukin-10 （IL-10）
