内毒素对牙龈成纤维细胞mCD14表达的影响

The effects of lipopolysaccharide on mCD14 expression in human gingival fibroblasts in vitro

目的:研究mCD14在牙龈成纤维细胞的膜表面分布及内毒素对mCD14在牙龈成纤维细胞膜表面表达的影响。方法:组织块法培养人牙龈成纤维细胞,利用免疫组化和Western blot方法研究mCD14在牙龈成纤维细胞的膜表面分布,同时观察内毒素对mCD14在牙龈成纤维细胞膜表面表达的影响。结果:免疫组织化学染色实验和蛋白印迹实验结果均表明mCD14在牙龈成纤维细胞的膜表面表达阳性,同时LPS可增强膜表面的mCD14的表达。结论:本实验表明正常人牙龈成纤维细胞可表达mCD14,LPS可以上调膜表面mCD14的表达,从而导致牙周损伤。

Objective：To study the distribution of CD14 in human gingival fibroblasts （HGFs） and the effects of lipopolysaeeharide（LPS） on mCD14 expression in HGFs in vitro. Methods：HGFs were in vitro cultured and exposed to LPS at 0. 1 - 10 μg/L. The expression of mCD14 in the cells was examined by immunohistochemistry staining and Western blot. Results：mCD14 was found on healthy human HGF cell membrane surface and LPS at 1 μg/ nd and 10 μg/ml increased the expression of mCD14 in 1 - 12 h exposure. Conclusion： LPS may regulate mCD14 expression of HGFs.