新基因mcpr1在小鼠牙胚发育中的时空表达和意义

The expression and function of mcpr1 gene during mouse tooth germ development

目的:探讨mcpr1在牙胚发生过程中的可能作用。方法:采用免疫组化LsAB法观察MCPR1在小鼠牙胚发育各个时期的表达分布及其变化情况。结果:MCPR1在小鼠牙胚各个时期均有表达,但各期分布模式有所不同,帽状期开始在成釉器上皮细胞表达,在釉结节内亦呈现强阳性表达,在分化成熟的成牙本质细胞及成釉细胞内呈现出强阳性表达。结论:mcpr1可能通过上皮-间充质之间的信号传递及相关基因的相互调控,参与成釉器的成熟及牙胚的发育。

Objective：To observe the temporal and spatial expression and function of mcprl gene during murine tooth germ development. Methods ：The expression of MCPR1 at different stages of mouse tooth germ were detected by immunohistochemical staining. Results：MCPR1 expression was detected at all stages of tooth germ, but the distribution patterns at various stages were different. It indieated that the temporal and spatial expression pattern of MCPR1 ,luring routine tooth germ development was specific. Conclusion： mcpr might play an important role in modulating the differentiation and mature of enamel organ.