变形链球菌临床株F-ATPase亚基基因uncEBF在mRNA水平的表达

F-ATPase subunit uncEBF mRNA expression in Streptococcus mutans clinical isolates

目的:研究变形链球菌临床分离株耐酸因子F-ATPase c、a、b亚基联合基因uncEBF在mRNA水平的表达差异,探讨基因表达水平与细菌耐酸力以及uncEBF基因型的关系。方法:应用半定量RT-PCR两步法和凝胶成像系统定量软件分析,分别对18株来自不同基因型和不同耐酸力变链菌的uncEBF基因表达水平进行评价,以SPSS11.0软件分析和比较mRNA表达的差异。结果:不同基因型及不同耐酸力菌株un-cEBF的mRNA表达水平不同(P<0.05),表现为A基因型uncEBF表达高于B基因型,高耐酸力菌株un-cEBF表达高于低耐酸力菌株。结论:变链菌不同基因型uncEBF基因的表达水平不同,表达水平高低与菌株的耐酸力相关。

Objective：To investigate the gene expression variety of different genotype of F-ATPase subunit uncEBF in Streptococcus mutans （S. ,mutans） and to evaluate the relationship among uncEBF gene expression levels, genotypes and the acidurance ability of S. mutans. Methods：The relative expression quantity of uncEBF gene against the housekeeping gene recA was determined by the two-step method of semi-quantitative RT-PCR in 18 clinical isolates of S. mutans（7 with genotype A uncEBF and 11 with genotyp B, 10 with high acidurance and 8 with low）. A gel documentation system and QUANTITY ONES software were used to assay the data. Results：uncEBF mRNA expression level in the isolates with genotype A uncEBF was higher than that in those with genotype B（ P 〈 O. 05）. uncEBF mRNA expression in the isolates of S. mutans with high ariduranee was higher than that with low acidurance（ P 〈0.05）. Conclusion：uncEBF gene of S. mutans clinical strains existes polymorphism at mRNA expression level. The increased expression of uncEBF gene may be ralated to the increased acid tolerance of S. mutans.