变形链球菌母子传播与其对牙面初始粘附关系的研究

Relationship between Mutans streptococci transmission from mothers to children and initial adherence properties

目的:探索变形链球菌母子传播与其对牙面初始粘附能力的关系.方法:首先以AP-PCR检测Mutans streptococci(MS)在20对3～4 岁儿童和母亲之间的传播,并从母亲口腔中筛选出传播株和非传播株;再采用同位素标记法,检测传播株和非传播株对SHA的粘附差异,同时对其表面蛋白粘附功能区spap-a和spap-pv分别用限制性内切酶haeⅢ和AluⅠ进行RFLP分析.结果:传播株较非传播株具有较弱的粘附力 (P<0.01),spap-a和spap-pv经酶切后分别呈现的3 种和2 种基因型在传播和非传播人群的MS中分布不同(P<0.01).结论:初始粘附能力弱的菌株被传播的可能性较大,不同MS株传播能力的差异可能与其表面蛋白A区,PV区的基因多态性有关.

Objective：To investigate the relationship between Mutans streptococci （MS） transmission from mothers to children and its initial adherence properties. Methods：200 MS isolates were genotypied by AP-PCR to demonstrate transmission between 20 pairs of mother and child aged 3 -4 years, and to detect the transmitted strains and non-transmitted strains of mothers. Then the adherence of the strains to salivary coated hydroxyapatite beads （SHA） were determined by 3H- thymidine incorperation assay. A restriction fragment length polymorphism （RFLP） study of the different regions（spap-a,spap-pv） of the spap were undertaken by endonuclease hae Ⅲ and Alu Ⅰ respectively. Results：The transmitted strains showed weaker adherence properties than the non-transmitted strains （P 〈 0.01 ） , different RFLP patterns of spap were observed among MS strains from different populations. Conclusions： A MS strain owning a poorer initial adherence property may have greater possibility of transmission. The different ability of transmission of MS strains may be related to the diversity of A-region and PV-region of spap.