摘要
介绍荧光的产生、荧光寿命的产生机理以及荧光寿命测量的基本原理.设计了一种利用直接记录法(光子计数法)测量农药荧光寿命的测试系统.该系统针对待测样品的特性,选用了相应的脉冲光源、光学元件和半导体探测器等器件,优化了各器件的工作参数,进行了简易而又科学的模块化设计,并对西维因农药的荧光寿命在无激励光干扰情况下进行了实际测试,测得了西维因溶液在500 μg/L浓度时的荧光衰减曲线和荧光寿命(0.30~0.40 ns).结果表明,该系统具有结构简易、操作方便的优点,能测量100 ps级的荧光寿命,适合于对能发荧光的农药进行荧光寿命的定量测量.
The generation of fluorescence, the mechanism of fluorescence lifetime and the basic measuring principle for fluorescence lifetime are introduced. A test system for fluorescence lifetimes of pesticides is designed by means of the direct record method (the Photon Counting Technique). According to the natures of the samples under test, a corresponding pulsed light source, optical elements, a semiconducting detector, etc. were chosen. All the working parameters such as the pulsed period of the excitation light source were reasonably optimized, a simple modular design was employed, the fluorescence lifetime of carbaryl solution was measured without the interference of the excitation light, and the fluorescence decay curves and the lifetime of 300~400 ps (FWHM) were obtained as the concentration of the carbaryl solution is 500μg/L. The results indicate that the system has the virtues of the simplicity in structure and convenience to operate, and can measure lifetimes as short as the order of 100 ps. It is suitable for the quantitative measurement fluorescence lifetime of pesticides which can emit fluorescence.
出处
《应用光学》
CAS
CSCD
2005年第6期28-31,共4页
Journal of Applied Optics
基金
国家自然科学基金(60272027)
黑龙江省自然科学基金(F0312)
关键词
荧光寿命测试系统
荧光寿命
光子计数法
西维因农药
measuring system of fluorescence lifetime
fluorescence lifetime
photon counting technique
carbaryl pesticide