子宫内膜组织与腹膜组织共同培养模型的建立

Co-culture of intact Peritoneum Explants and Endometrial Tissue

目的:探讨如何建立人腹膜组织与子宫内膜组织共培养的离体实验模型,以利于今后对子宫内膜异位症发病机制的研究。方法:①取前腹膜,将其剪成0.5 cm×0.5 cm大小,贴于预先包埋了鼠尾胶的24孔培养板中,将处理过的子宫内膜取约15 mg(湿重)滴在已经贴壁的腹膜组织上共培养。②HE染色,扫描电镜检查和免疫组化方法进行观察、测定。结果:①离体培养的腹膜间皮细胞与活体组织相比,其形态和细胞学特征无显著改变,而且大多数的间皮细胞是存活、完整的。②子宫内膜可以粘附于腹膜,而且共培养1小时,24小时均有粘附。结论:腹膜组织与子宫内膜组织共培养后可以作为子宫内膜异位症的研究模型。

Objective：To establish the model of co- culture tissue of peritoneum and endometrium in vitro for the study of endometriosis. Methods： 1. Explants of intact human peritoneum from the anterior abdominal wall were cultured with whole fragments of mechanically dispersed endometrium. 2. Adhesion of endometrial fragments was studied by histologic examination and scaning electron microscopy. Results： 1. After incubation, the mesothelium was in most areas, immunohistochemical staining revealed that cytokeratin vimentin were still present in the cell cytoplasm. 2. Endometrium was identified attached to the surface of the peritoneum. Conclusions： Explants of intact human peritoneum issuitable for the model of tissue culture.