血管内皮生长因子受体KDRmRNA和flt-1 mRNA与子宫内膜异位症相关性研究

Expression of Vascular Endothelial Growth Factor Receptors flt-1 and Kinase Insert Domain-contaning Receptor(KDR) mRNA in Endometriosis

目的:探讨血管内皮生长因子受体(VEGFR)中的fms样酪氨酸激酶受体(flt-1)和胎肝激酶受体(KDR)在子宫内膜异位症(内异症)患者异位及在位子宫内膜组织中的表达及其意义。方法:采用RT-PCR技术,从核酸水平检测53例内异症患者(44例卵巢子宫内膜异位囊肿,30例内异症在位子宫内膜组织)中的VEGF受体KDRmRNAf、lt-1mRNA的阳性表达率及相对表达强度,以40例正常子宫内膜组织作为对照。结果:与对照组比较,研究组KDRmRNA和flt-1mRNA的阳性表达率高于对照组,差异有显著性(P<0.05);研究组在位内膜增殖期与分泌期的阳性表达率、相对表达强度高于对照组子宫内膜组织,差异有显著性(P<0.05);不同月经周期在位内膜组织KDRmRNA和flt-1mRNA的表达、两组增殖期KDRmRNA相对表达强度高于分泌期flt-1mRNA,而flt-1mRNA分泌期相对表达强度高于KDRmRNA。结论:VEGF受体KDRmRNA和flt-1mRNA在内异症中有明显表达并呈周期依赖性。VEGF受体在内异症血管生成中的作用,可能与内异症远处转移、侵袭、种植相关。

Objective, To detect the expression of vascular endothelial growth factor receptors （VEGFR）flt- 1 and KDR mRNA in the ectopic and eutopic endometrium of patients with endometriosis. Methods. 44 specimens of ovarian endometriorna and 30 eutopic endometrium were biopsied from 53 patients with endometriosis; another 40 specimens of endometrium were biopsied from women without endometriosis. Reverse transcript- polymerase chain reactiorl （RT- PCR） was utilized to examine expression and the level of KDR mRNA and fit- 1 mRNA in the endometrium specimens. Results： Among endometriotic lesions, KDR mRNA and fit - 1 mRNA expressed higher level than women without endometriosis. The expression of KDR protein was higher in the secretory phase than that in proliferative phase, on the contrary, fit- 1 protein expression was higher in proliferative phase than that in secretory phase. Conclusions,The VEGF receptors KDR mRNA and fit- 1 mRNA highly expressed in endometrial endothelial cells of endometriosis, and showed a pronounced menstrual cycle- dependency, the VEGFR＇ s function in angiogenesis of endometriosis might be related to the transfer of the disease,