松花粉对前列腺增生大鼠的干预效应

Interventional effects of pine pollen in rats with hyperplasia of prostate

目的:观察松花粉对大鼠前列腺增生的干预效果。方法:实验于2003-07/09在解放军总医院微量元素室完成。选用清洁级3个月龄雄性SD大鼠24只,随机分为正常对照组、淀粉安慰剂组、松花粉组,8只/组。正常对照组始终饲喂普通饲料,淀粉安慰剂组、松花粉组每天投料前分别喂以安慰剂淀粉片和松花粉片3片,片剂加饲料的总重量与正常对照组饲料量保持一致。适应2周后,正常对照组每天肌肉注射橄榄油1mL/kg,淀粉安慰剂组、松花粉组每天肌肉注射橄榄油配制的丙酸睾酮应用注射液4mg/kg,持续2周,并始终保持相同饲喂方法。4周后全部麻醉处死,进行各项指标检测。血清睾酮及雌二醇采用化学发光法检测,血生化指标用原子吸收分光光度法测定,红细胞及组织中超氧化物歧化酶采用黄嘌呤氧化酶法检测,血清及组织中丙二醛采用硫代巴比妥酸比色法测定。结果:实验纳入大鼠24只,全部进入结果分析。①前列腺指数及病理切片观察结果:松花粉组前列腺重量及前列腺指数均低于淀粉安慰剂组[(1062.3±91.9),(1127.3±111.2)mg;(3.18±0.31),(3.26±0.31)mg/g;P<0.05]。病理切片观察显示,淀粉安慰剂组前列腺腺体排列密集,上皮细胞呈复层或假复层,乳头状增生的百分率明显高于松花粉组,间质小血管扩张充血。而松花粉组腺体体积明显缩小,上皮细胞呈单层立方或扁平状,乳头状增生明显减少。②生化指标检测结果:与正常对照组比较,松花粉组血糖、总胆固醇、三酰甘油、尿素氮、雌二醇均明显降低[(6.43±2.04),(4.07±1.38)mmol/L;(1.78±0.10),(1.49±0.17)mmol/L;(1.11±0.30),(0.61±0.10)mmol/L;(9.42±0.97),(7.44±0.79)mmol/L;(253.23±40.55),(199.12±37.09)nmol/L;P均<0.05];与淀粉安慰剂组比较,松花粉组睾酮含量显著降低[(11.85±8.25),(8.85±2.69)nmol/L,P<0.05]。③血清及组织中微量元素锌、铜检测结果:与正常对照组比较,松花粉组血浆中锌、铜含量均明显降低[(1.60±0.20),(1.34±0.16);(1.49±0.16),(1.17±0.05)mg/L;P均<0.05],前列腺中锌、铜含量均明显升高[(130.2±29.9),(187.9±56.0);(0.86±0.12),(1.07±0.39)mg/L;P均<0.05]。④抗氧化指标检测结果:与正常对照组比较,松花粉组肝脏及前列腺中超氧化物歧化酶活性均明显升高[(3.18±0.57),(3.30±0.64);(2.90±1.41),(4.67±1.66)μkat/g;P均<0.05],而红细胞中超氧化物歧化酶活性明显降低[(0.38±0.05),(0.30±0.04)μKat/g;P<0.05];肝脏及前列腺中丙二醛的含量均明显下降[(7.40±1.87),(5.85±1.20);(6.24±2.31),(5.67±2.30)nmol/g;P均<0.05]。结论:松花粉不仅能够降低机体雌、雄激素水平,调节微量元素代谢和超氧化物歧化酶的活性,抑制前列腺增生,同时其有效成分还可以通过非激素途径在细胞水平上发挥选择性抑制作用。

AIM： To study the effects of pine pollen on the intervention in rats with hyperplasia of prostate. METHODS： The experiment was conducted at Room of Microelement, General Hospital of Chinese PLA from July to September 2003. Twentyfour clean-grade SD male rats aged 3 months were selected, and divided randomly into normal control group, starch placebo group and pine pollen group with 8 rats in each group. The rats in the normal control group were fed with common feed all the time. Those in the starch placebo group and pine pollen group were treated with 3 placebo starch tablets and 3 pine pollen tablets, respectively every day before giving feed. The total content of tablet and feed was the same to that in the normal control group. After 2-week adaptation, those in the normal control group were injected with 1 mlJkg olive oil every day into muscle. Those in the starch placebo group and the pine pollen group were injected with 4 mg/kg androtest parenteral solution dispensed by olive oil into muscles every day for 2 weeks, and the same feed way was kept. Four weeks later they were killed after anesthesia, and the examination on every indexes was performed. Serum homosteron and destradiol were detected with chemiluminescence. Blood biochemical index was measured with atom absorption spectrophotography. Superoxide dismutase （SOD） in erythrocyte and tissue was detected with xanthine oxidase method. Malondialdehyde （MDA） in serum and tissue was tested with thiobarbituric acid （TBA） colorimetric method. RESULTS： Totally 24 included rats in the experiment were involved in the result analysis. ① Prostate index and observation result of pathological section： The prostate weight and index in the pine pollen group were lower than those in the starch placebo group [（ 1 062.3±91.9）, （ 1 127.3±111.2） mg; （3.18±0.313, （3.26±0.31） mg/g;P 〈 0.05]. The pathological section showed that in the starch placebo group the prostate gland arranged very close; the epithelial cells appeared stratified shape or sham stratified shape. The papillary hyperplasia ratio was much higher than that in the pine pollen group with ectasia and hyperemia of small vessels of mesenchymal. While the gland volume in the pine pollen group shortened significantly. The epithelial cells showed simple layer cube or thin and fiat shape, and the papillary hyperplasia decreased significantly. ② Biochemical index： Compared with the normal control group, the glucose, total cholesterol, triacylglycerol, urea nitrogen and destradiol in the pine pollen group reduced significantly [（6.43±2.04）, （4.07±1.38） mmol/L; （1.78±0.10）,（1.49±0.17） mmol/L;（1.11±0.30）,（0.61±0.10） retool/L; （9.42±0.97）, （7.44±0.79） mmol/L; （253.23±40.55）, （199.12±37.09） nmol/L; P 〈 0.05]. Compared with the starch placebo group, the homosteron content in the pine pollen group fell down significantly [（11.85±8.25）, （8.85±2.69）nmol/L,P 〈 0.05]. ③ Zinc and copper in serum and tissue： Compared with the normal control group, the contents of zinc and copper in plasm decreased significantly [（ 1.60±0.20）, （1.34±0.16） ; （ 1.49±0.16）, （1.17±0.05）mg/L;P 〈 0.05]. The contents of zinc and copper in prostate increased significantly [（130.2±29.9）, （187.9±56.0） ; （0.86±0.12）, （ 1.07±0.39）mg/L;P 〈 0.05]. ④ Anti-oxidizing index： Compared with the normal control group, the activities of SOD in the liver in pine pollen group and prostate were all increased significantly [（3.18±0.57）, （3.30±0.64） ; （2.90±1.41）, （4.67±1.66） μkat/g;P 〈 0.05], while the activities of SOD in erythrocyte lightened obviously [（0.38±0.05）, （0.30±0.04） μkat/g;P 〈 0.05]. The content of MDA in the liver and prostate decreased remarkably [（7.40±1.87）, （5.85±1.20） ; （6.24±2.31 ）, （5.67±2.30） nmol/g;P 〈 0.05]. CONCLUSION： The pine pollen not only can decrease the levels of body estrin and androgen, adjust microclement metabolism and activity of SOD, inhibit the hyperplasia of prostate, but its effective component can play selective inhibition on cell level through non-hormone way.