摘要
【目的】探讨染矽尘大鼠肺组织转化生长因子-β1(TGF-β1)蛋白水平的改变在矽肺发病中的意义。【方法】将96只大鼠随机分为对照组、染矽尘组,采用一次性气管内注入矽尘法建立矽肺动物模型。染尘后按不同时间点取大鼠肺组织制作组织芯片,链菌素-过氧化物酶法检测TGF-β1在肺组织中的表达,用Image-ProPlus图像分析系统对TGF-β1表达作定量分析。【结果】对照组大鼠有极少量TGF-β1在肺组织内表达;染矽尘组大鼠肺组织TGF-β1表达明显增强,TGF-β1阳性细胞积分光密度增加,7 d时达高峰,与对照组比较有显著性差异(P<0.05),14 d时开始下降,至28 d时两组差异无显著性(P>0.05)。【结论】矽尘可以诱导肺组织过度表达TGF-β1,TGF-β1可能与早期矽肺纤维化病变有关。
[Objective] To study the expression of transforming growth factor-β1 （TGF-β1） in lung tissue of silica-treated rat. [Methods] The 96 experimental rats were randomly divided into control and silica group, 1ml silica （50 g/l） was injected intratracheally in rats of silica group. Samples were collected on the 1, 3, 7, 14, 21, 28 d after injected silica. Expression of TGF-β1 on tissue microarray were detected with Streptavidin/peroxidase immunohistochemistry and quantified by Image-Pro Plus. [Results] TGF-β1 expressed very little in the macrophages of control group while it markedly increased in lung tissue of silica group. TGF-β1 positive cells reached the peak on the 7 d. There was significant difference of TGF-β1 integrated optical density between silica group and control group （P 〈 0.05）. Then the expression decreased from the 14 d. It was still higher than control group on the 28 d, but the difference was not significant. [Conchusion] Silica dioxide can induce the over expression of TGF-β1 in lung tissue of silica-treated rat.
出处
《武警医学院学报》
CAS
2005年第6期454-456,460,F0003,共5页
Acta Academiae Medicinae CPAPF
基金
国家自然科学基金资助项目(30070654)
