免疫抑制剂对IL-2致痫大鼠大脑皮质内一氧化氮合酶表达的影响

EFFECTS OF IMMUNOSUPPRESSANTS ON NOS EXPRESSION IN CEREBRAL CORTEX OF RATS WITH SEIZURE INDUCED BY IL-2

为探讨免疫抑制剂对致痫大鼠大脑皮质内一氧化氮合酶(NOS)表达的影响,本实验采用Western blot法和图像分析技术观察了经免疫抑制剂预处理后致痫大鼠行为的改变与大脑皮质内NOS表达的变化。结果发现:(1)侧脑室注射白细胞介素-2(IL-2)可引起大鼠明显癫痫发作症状,可使大脑皮质内NOS表达较生理盐水对照组明显升高(P<0.05);(2)环胞素-A(cyclos-porine,CsA)预处理可减轻癫痫发作程度,并使NOS表达明显下降(P<0.05);(3)糖皮质激素(glucocorticoids,GC)注射组大鼠呈抑制状态,且其皮质内NOS表达较生理盐水组显著下降(P<0.05)。结果提示,IL-2具有致痫效应和促进NOS表达的作用,免疫抑制剂具有明显的抑制NOS表达的作用和抗痫效应。

In order to investigate the effect of immunusuppressants on NOS in cerebral cortex of epileptic rats, the changes of NOS expression in rats with seizures following the pretreatment of immunosuppressants were studied by means of Western blot anti computer-assisted image analysis, and the behavior changes were observed. The results demonstrated that ： （ 1 ） seizure developed after injecting IL-2 into the lateral ventricle of rats. and the expression of NOS in the cerebral cortex was significantly higher than that of the normal saline control group （ P 〈 0, 05 ） ： （2） seizure was restrained and the expression of NOS in the cerebral cortex was decreased significantly in the cyclosporine ＋ IL-2 group （ P 〈 0, 05 ） cnmpared with the IL-2 group ; （ 3 ） the rats were in a suppressive state in the glucocorticoids group and the expressinn of NOS in the cerebrat cortex was greatly decreased, compared with the normal saline control group（ P 〈 0.05 ）, These data suggest that IL-2 could induce seizure and promnte the expression of NOS. Immunosuppressants could have the function of restraining the seizure anti significantly reduce the expression of NOS.