杂交油菜种子纯度的RAPD检测研究

Study on Hybrid Rapeseed Seed Purity Test with RAPD

在简化油菜DNA提取方法的基础上,建立了适用于杂交油菜种子纯度检测的RAPD技术分析系统,从814条随机引物中筛选获得了2条可同时稳定区分CM S杂交油菜品种秦优7号及其亲本的引物;经过大量的不同来源油菜个体的验证,证明了该方法具有很好的重复性、准确性和可靠性;与酯酶同工酶法比较,发现利用RAPD技术能够检测酯酶同工酶法难以鉴别的杂交种及其亲本系.因此,选择合适引物、建立良好的反应体系、简化DNA提取程序、降低分析成本等,可使RAPD技术实用化.

Hybrid rapeseed seed purity test system with RAPD technique was established on a simple and rapid method of DNA extraction on rapeseed. 2 primers were singled out from 814 primers （10 mer） to distinguish “Qinyou No 7” （CMS hybrid,Brassica napus L. ） and its parents obviously. The repeatability,veracity and reliability of the test technique was proved to be good by experiment with large numbers of indi vidual plant of different sources. Comparing with method of esterase isoenzyme shown that RAPD technique can distinguish the hybrid and their parents that esterase can not. It was shown that RAPD technique can be used practically by selecting appropriate primer,establishing suitable reaction system,simplifying the program of DNA extraction and reducing the cost of test.