骨蚀宁对激素性股骨头坏死家兔局部rBF、TNF及显微结构的影响

Effects of Gushining Decoction on rBF,TNF and Pathology in Rabbits with Steroid-induced Avascular Necrosis of Femoral Head

目的:探讨骨蚀宁治疗激素性股骨头坏死的作用机制。方法:选择马血清与甲基强的松龙造成兔激素性股骨头坏死模型,设立对照组、模型组、仙灵骨葆组、骨蚀宁组,测定其血中局部组织血流量(rBF)、肿瘤坏死因子(TNF),同时取骨在光镜下进行常规病理检查观察。结果:与模型组比较,仙灵骨葆组与骨蚀宁组rBF明显升高(P<0.01),但两组之间无明显差异(P>0.05)。与模型组比较,仙灵骨葆组与模型组TNF含量差别不大(P>0.05),而骨蚀宁组升高明显,差异有显著性(P<0.05)。与仙灵骨葆组比较,骨蚀宁组TNF明显降低,差异有显著性(P<0.05)。与模型组比较,仙灵骨葆组和骨蚀宁组空骨陷窝百分比均有减少,差异有显著性(P<0.01)。但均未达正常,组间比较无显著性差异(P>0.05)。骨蚀宁组股骨头骨小梁和骨细胞的损害明显减轻。结论:骨蚀宁可通过改善股骨头局部组织血流量、抗炎性反应以及保护和促进骨细胞再生达到治疗激素性股骨头坏死的作用。

Objective： To observe effects of Gushining decoction on steroid-induced avascular necrosis of femoral head in rabbits, and to study further its mechanism. Method： Following the injection of large dosage of horse serum and methylprednisolonum,accumulation of lipid and necrosis of osteocytes in the femoral heads were seen in the model group of rabbits. Forty Chinese rabbits were employed and divided randomly into 4 groups, namely control group, model group, Xianling gubao group and Gushining group. The rBF and TNF were measured after 4 weeks, and also HE staining and pathological section were done in order to study the changes of histopathology under microscope. Result： Comparing with those in the control group, the rBF of the model group and Xianling gubao group decreased respectively while TNF increased （P〈0.01）, and also rate of empty lacuna in the femoral heads decreaced in the model group and Xianling gubao group （P〈0.01）, but both between the model group and Xianling gubao group had no difference （P〉0.05）. Impairment of bone trabeculae and bone cells induced by steroid was remarkably reduced in the Gushining group. Conclusion： Gushining decoction may treat disease of steroid-induced avascular necrosis of femoral head through improving microcirculationg of femoral head, reducing or restraining the inflam-mative cells, and protesting and promoting bone formation.