二丙酰基莲心碱对猪冠状动脉条的影响

Effect of O,O-Dipropionyl-liensinine on the Strips of Porcine Coronary Artery

目的:观察二丙酰基莲心碱拮抗KC l、乙酰胆碱和组胺所致猪冠状动脉条收缩的作用。方法:体外平滑肌实验方法,观察二丙酰基莲心碱对KC l、乙酰胆碱和组胺所致猪冠状动脉条收缩曲线的影响以及在无Ca2+克氏液中,对组胺引起猪冠状动脉条第一相收缩和Ca2+引起第二相收缩的影响。结果:不同剂量二丙酰基莲心碱均可使KC l、乙酰胆碱和组胺所致冠脉条收缩量效曲线呈非竞争性拮抗作用,对冠脉条第一相和第二相收缩都有明显的抑制作用。结论:二丙酰基莲心碱具有扩张冠脉的作用,此作用与拮抗细胞内钙的释放和抑制外钙内流有关。

Objective： To observe the antagonism of on contraction of porcine artery strips induced by KCl, acetyl choline and Histamine respectively. Method： Using experimental method of porcine coronary arterial smooth muscle in vitro, the effect of O, O-Di propionyl-liensinine on pig arteria coronary contraction induced by KCl, acetyl choline and histamine, and the effect on the intracellular Ca^2＋ -dependent contraction induced by histamine and the extracellular Ca^2＋ -dependent contraction induced by CaCl2 in Krebs solution are observed. Result： Different dosage of O, O-Dipropionyl-liensinine had noncompetitively antagonizing effect on KCl, acetylcholine and Histamine cumulative dose-response curves. O, O-Di propionyl-liensinine and ver oinhibited intracellular Ca^2＋ -dependent contraction induced by Histamine; and show relatively small effect on the extracellular Ca^2＋ -dependent contraction induced by CaCl2. Conclusion： O, O-Dipropionyl-Liensinine has a significant relaxing effect on contraction of coronary artery, and has an antagonistic effect on the two kinds of contractions.