原发性肝癌趋化因子的表达及其相关性研究

Study on the Expressions of IL-8,MCP-1,MIP-1α and Their Relationships with Primary Hepatocarcinoma Tissues

【目的】研究原发性肝癌(PHC)及其癌旁肝组织IL-8、MCP-1和MIP-1α表达,并探讨其相互关系及临床病理意义。【方法】47例PHC及其癌旁组织经10%中性福尔马林固定后制作石蜡包埋切片,趋化因子染色为ABC免疫组化法。【结果】PHC组织IL-8、MCP-1、MIP-1α阳性率及其评分(51.1%,2.0±1.9;61.7%,2.2±1.5;51.1%,2.2±1.2)明显高于癌旁上皮(6.4%,0.3±0.9;10.6%,0.6±1.1;0%,0.3±0.6),均有高度显著差异(P<0.01);高分化、无转移、无癌栓、结节型及肿块最大径<5 cm PHC三者评分低于低分化、有转移、有癌栓、巨块型及肿块最大径≥5 cm病例,但仅IL-8在大体形态和肿块最大径上有显著差异(P<0.05)。【结论】IL-8、MCP-1和MIP-1α表达可能是反映PHC发生发展、生物学行为和预后的重要参照物。

[Objective]To study on the expressions of IL-8, MCP-1 and MIP-la and detect their relationships and clinicopathological significances in PHC tissues. [Methods]Immunohistochemical method of avidinbiotin complex was used for the expressions of IL-8, MCP-1 and MIP-1a on the formalin-fixed and routinely paraffin-embedded sections of 47 cases with PHC and pericancerous liver tissues. [Results]The positive rates and scorings of IL-8, MCP-1 and MIP-1a were significantly higher（P〈0.01） in the cases of PHC than those of pericancerous liver tissues（IL-8：51.1% vs 6.4%, 2.0±1.9 vs 0.3±0.9; MCP-1：61.7% vs 10.6%, 2.2±1.5 vs 0.6±1.1; MIP-1α：51.1% vs 0%, 2.2±1.2vs 0.3±0.6）. The scorings showed lower in the cases of well-or middle-differentiation, without-metastasis, without cancer emboli, nod shapes and maximal diameter of mass〈5cm than those of poor-differentiation, with metastasis, with cancer emboli, mass shapes and maximal diameter of mass≥5cm in PHC tissues, they showed statistic differences among the gross shape and maximal diameter of mass in IL-8 expression （ P 〈0.05）. [Conclusion]The expression of IL-8, MCP-1 and MIP-1α may be important marker in reflecting the carcinogenesis, progressions, biological behaviors and prognosis of PHC.