蛇床子素对大鼠的抗炎作用和机制(英文)

Anti-inflammatory Effect and Mechanism of Osthole in Rats

目的:研究蛇床子素(Ost)的抗炎作用及机制。方法:用角叉菜胶诱导大鼠足肿模型。研究Ost对炎症的影响。NOS活性用NADPH黄递酶染色法,NO含量用Griess重氮化反应法,MDA用TBA荧光法测定,前列腺素类含量的测定通过紫外可见光分光光度法,经过0.5 mol/L氢氧化钾甲醇溶液,在50℃的温度中为异构后,在波长为278 nm处检测。结果:给角叉菜胶4小时后NO的含量明显提高,而蛇床子素各组对NO的含量有明显抑制作用。从单纯给角叉菜胶组NO为524.4±58.7μmol/L到蛇床子素50 mg/kg、100 mg/kg NO的含量分别为461.5±68.8,353.8±111.0/μmol/L NO-2,表明对炎症因子NO的抑制作用呈剂量依赖性(P<0.05)。蛇床子素100 mg/kg对致炎大鼠NOS活性有明显抑制效应,其水平接近空白对照组。与此同时,结果显示蛇床子素也抑制致炎大鼠的脂质过氧化产物———MDA的产生。蛇床子素50,100 mg/kg显著抑制角叉菜胶致炎大鼠前列腺素类水平。结论:通过常见的实验方法表明蛇床子素具有抗炎作用,其抑制作用可能是通过抑制细胞钙内流和提高环鸟苷酸的水平而影响PG,NO,MDA等含量和cNOS的活性。

Objective： To investigate the anti-inflammatory effect and mechanism of osthole（Ost）. Methods： Carrageenan -induced hind paw edema in rats were prepared. The nitric oxide synthase（NOS） activity was measured by NADPH -diaphoras stain assay, nitric oxide（NO） content by Griess diazotization assay, malondialdehyde（MDA） content by Thibabituric acid mehtods. And PG content was assaied by Uv -vis spectrophotometry with 278 nm after 0.5 mol/L KOH methanol reagent dissimilating. Results： The increase in NO 2 observed 4h after carrageenan administration was inhibited by Ost from 524.4±58.7/μmol/L-1NO 2 for carrageenan alone to 461.5±68.8,353.8±111.0/μmol/L NO 2 for, respectively, carrageenan administered in combination with 50 mg/kg or 100 mg/kg Ost. It suggested Ost suppress inflammatory responses on NO levels in a dose -dependent manner（P＜0.05）. In the presence of Ost 100 mg/kg, NOS activities remained at near blank control levels. Meanwhile results showed reduced malondialdehyde production （MDA） in the presence of Ost, which is one of the byproducts of lipid peroxidation. Ost 50,100 mg/kg markedly suppressed the generation of PG in inflamed paws. Conclusion： Ost presents anti-inflammatory activities at the used models. The effects may be associated to a supression of content of PG, NO, MDA and cNOS activity by inhibition of calcium entry and elevating cGMP levels way.