太子参醇提物对大鼠组织和红细胞的抗氧化活性

Antioxidative activity of EPHPH on tissues and erythrocytes of rats

目的:研究太子参醇提物的抗氧化活性及其机制.方法:用.HO生成系统Fe2++抗坏血酸诱导大鼠心、肝、肾组织匀浆脂质过氧化,TBA比色法测定MDA含量;NBT还原法测酵母多糖A刺激大鼠中性粒细胞产生的O2-;分光光度法测H2O2诱发的大鼠红细胞溶血度.结果:6.7,13.4,26.8,53.5,107.0和214.0μg/L太子参醇提物不同程度抑制Fe2++抗坏血酸诱导的大鼠心、肝、肾MDA生成,其抑制心、肝、肾MDA生成的IC50分别为57.0,51.5及53.2μg/L,其量效关系均呈负相关,相关系数分别为-0.891,-0.883,-0.893(P均<0.001);不同程度抑制酵母多糖A刺激大鼠中性粒细胞生成O2-及红细胞氧化溶血,IC50分别为83.1和86.5μg/L,其量效关系均呈负相关,相关系数分别为-0.894和-0.901(P均<0.001).结论:太子参醇提物通过清除.HO,O.HO,O2-及H2O2而发挥抗氧化活性.

AIM： To study the antioxidative activity of the alcoholic extract from Pseudostellaria heterophylla Pax et Hoffm （EPHPH） and its mechanism. METHODS： The peroxidation in homogenates of heart and liver and kidney of rats was induced by HO generation system Fe^2＋ ascorbic acid. Malondialdehyde （ MDA ） was determined using TBA colorimetric method and superoxide （O2^- ） from zymosan-stimulated neutrophils of rats was measured by NBT reduction method. H2O2 -caused hemolysis of erythrocytes was measured spectrometrically. RESULTS： MDA formation in homogenates of heart and liver and kidney of rats induced by HO generation system Fe^2＋ ascorbic acid was inhibited differently by 6.7, 13.4, ：26.8, 53.5, 107.0 and 214.0 μg/L EPHPH, whose IC50 that inhibited MDA formation in heart, liver and kidney was 57.0, 51.5 and 53.2 μg/ L respectively, whose relation between quantity and efficiency showed all negative and whose relative coefficient was - 0. 891,- 0. 883 and - 0.893 respectively （ all P 〈 0.001）. O2^- generation from zymosan-stimulated neutrophils of rats was inhibited distinctly by EPHPH, whose IC50 that inhibited O2^- generation was 83.1μg/L, whose relation between quantity and efficiency showed negative and whose relative coefficient was -0.894（ P 〈0.001 ）. H2O2^- caused hemolysis of erythrocytes was inhibited distinctly by EPHPH whose IC50 that inhibited hemolysis of erythrocytes was 86.5 μg/L, whose relation between quantity and efficiency showed negative and whose relative coefficient was -0.901 （ P 〈0. 001 ）. CONCLUSION： EPHPH exerts its effect on antioxidation by scavenging HO,O2^- and H2O2 in the biological system.