CD69在慢性乙肝患者CD4^+、CD8^+T淋巴细胞的表达

Expression of CD69 in CD4^+ and CD8^+ T lymphocytes in chronic hepatitis

目的:观测慢性乙肝患者外周血CD4+、CD8+T淋巴细胞早期激活标志CD69的表达。方法:静脉采集慢性乙肝患者普通肝素抗凝血2ml,在植物血凝素(PHA)20μg/ml条件下进行全血培养,4h后用双色免疫荧光标记流式细胞术检测CD4+、CD8+T淋巴细胞CD69的表达。结果:正常人外周血只有很少量CD4+与CD8+T淋巴细胞表达CD69分子,经PHA刺激后CD4+与CD8+T淋巴细胞CD69的表达率明显升高,较刺激前有统计学差异(P<0.01)。同样,慢性乙肝患者在激活前后也有统计学差异(P<0.01);慢性乙肝患者在PHA刺激前有一部分CD4+、CD8+T淋巴细胞表达CD69分子,明显高于对照组(P<0.01)。结论:本研究结果可能与乙肝患者体内病毒免疫紊乱有关系。

Objective： To study the expression of CD69 which is the nonage signal in CD4^＋ andCD8^＋T lymphocytes in the peripheral blood of chronic hepatitis patients. Methods： 2 ml venose blood from chronic hepatitis patients was collected, and nourished with phytohemagglutinin （PHA） （20 μg/ml）. Four hours later, the expression of CD69 was tested by flow eytometry （FCM）. Results： CD69 on the CD4^＋ and CDS^＋T cells was barely found in the healthy people, but was easily observed after the stimulation of PHA（P〈0.01）, as well as in chronic hepatitis patients; CD69 on part of the CD4^＋ and CD8^＋ T cells could be easily detected in chronic hepatitis patients, which was evidently higher than that in the healthy people. Conclusion：This result may be relevant to the immune tolerance in hepatitis B virus（HBV）.