短暂性脑缺血发作患者一氧化氮合酶基因碱基重复序列多态性研究

Study of a 27-bp variable number of tandem repeat polymorphism of the endothelial nitric oxide synthase gene in patients with transient ischemic attack of brain

目的探讨内皮细胞型一氧化氮合酶(eNOS)基因碱基重复序列(VNTR)多态性与短暂性脑缺血发作(TIA)的关系。方法应用聚合酶链反应(PCR)技术,检测112例TIA患者的基因型,并与104例对照组比较。结果老年TIA组和非老年TIA组eNOS基因ab基因型的频率差异无统计学意义(分别为26.2%和23.5%)(P>0.05),对照组中老年组和非老年组eNOS基因ab基因型的频率差异无统计学意义(分别为15.7%和16.9%)(P>0.05),TIA组eNOS基因ab基因型的频率(25.0%)明显高于对照组(16.3%),a等位基因的频率(14.3%)也明显高于对照组(9.2%),且差异有统计学意义(P<0.05)。颈内动脉系统TIA组eNOS基因ab基因型的频率(28.8%)和a等位基因的频率(17.4%)均明显高于椎基底动脉系统TIA组(19.5%和9.7%)和对照组(16.3%和9.2%),P<0.05。结论ab基因型与颈内动脉系统TIA有相关性。

Objective To investigate the association between a 27-bp variable number of tandem repeat（VNTR） polymorphism of the endothetial nitric-oxlde synthase （eNOS）gene and transient ischemic attack （TIA） of brain. Methods Polymerase chain reaction was performed for the genotypes of all patients and control subjects. Data were compared between patients and control subjects using X^2test. Results The genotype ab distribution and allele a frequencies of eNOS gene were significantly higher in TIA group（25.0% ,14.3%） than in control group（16.3% ,9. 2%） （P〈 0. 05）. The genotype ab distribution and allele a frequencies of eNOS gene were significantly higher in internal carotid artery group（28.8% ,17.4%） than in basilar artery group （19.5% ,9. 7%） ,P〈0.05. Conclusions There is an association between the VNTR polymorphism of eNOS in intron 4 and TIA. The allele a of eNOS may be an independent risk factor for ischemlc cerebral vascular disease.