血清骨保护蛋白水平与骨质疏松发病的关系

Serum osteoprotegerin level is closely associated with development of osteoporosis

目的探讨骨保护蛋白(OPG)在增龄、去卵巢小鼠和骨质疏松患者血清中的变化。方法应用双能X线骨密度仪和酶联免疫法分别测定增龄、去卵巢小鼠及骨质疏松症患者骨密度和血清OPG水平。结果去卵巢小鼠的骨密度显著低于增龄小鼠〔分别为(0.151±0.008)和(0.160±0.007)g/cm2,P<0.01〕,两组骨密度值均较成年鼠低(P<0.01);去卵巢小鼠血清OPG水平(20.57±7.00)pmol/L显著高于增龄小鼠(15.39±2.86)pmol/L,P<0.01;骨密度值与OPG浓度呈显著负相关(r=-0.246,P<0.05)。老年骨质疏松症患者血清OPG浓度男性和女性分别为(10.77±3.14)pmol/L和(10.56±2.56)pmol/L,均显著高于健康人〔男性和女性分别为(6.55±0.87)pmol/L(、9.19±1.73)pmol/L,P<0.01〕;血清OPG与年龄呈显著正相关(r=0.656,P<0.01)。结论血清OPG水平在骨质疏松小鼠模型和老年骨质疏松患者中均与骨密度或年龄密切相关,表明OPG浓度升高在老年绝经后骨质疏松的发病中具有重要作用。

Objective To illustrate the role of OPG in the development of osteoporosis, serum osteoprotegerin （OPG） and bone mineral density（ BMD） in aged, ovariectomized mouse models and in the aged patients were investigated. Methods Bone mineral density and serum OPG in the subjects with osteoporosis were detected by DEXA bone densitometer and OPG ELISA kits, respectively. The data were statistically analyzed with SPSS 12.0 software. Results The data showed that BMD of ovariectomized mice（0. 151 ±0. 008）g/cm^2 was significantly lower than that of aged mice （0. 160 ± 0. 007）g/cm^2 ,P〈0. 01. The BMD in both groups was lower than that in normal adult mice （P〈 0.01）. Correspondingly, serum OPG level in the aged（20.57±7.00）pmol/L and ovariectomized （15.39 ±2.86）pmol/L mice significantly increased in comparison with normal control（P〈0.01 ）. The BMD was found to be negatively correlated with serum OPG level（r=-0.246, P〈0.05,）. Furthermore, the significant increase of serum OPG was found in the aged patients with osteoporosis[female, （10. 77 ± 3.14） pmol/L, male, （ 10. 56 ±.56） pmol/L]compared with normal adult females （6. 55±0.87）pmol/L or the elders （9. 19±1.73）pmol/L,P〈0. 01. It was found that OPG level was positively correlated with age （r = 0. 656, P〈0. 01）. Conclusions The serum OPG level of the ovariectomized mouse is closely associated with the BMD and aging, OPG may play an important role in the development of postmenopausal osteoporosis or bone loss with aging.