鹿白细胞中抗菌肽的提取及抗菌活性鉴定

Extraction of Antimicrobial Peptides from Deer Leukocytes and ldentification of their Bactericidal Activities

应用NH4Cl裂解、超声波破碎、离子交换层析和蛋白质电泳等方法,对鹿白细胞中的抗菌肽成分进行了初步提取和分析,经平板抑菌试验初步检测了提取物的抗菌活性后,采用微量反应板法进行了最小抑菌浓度(MIC)测定.结果表明,在鹿白细胞颗粒中存在着天然抗微生物活性物质,其主要活性成分为多肽部分;MIC试验显示,该提取物对革兰氏阳性细菌、革兰氏阴性细菌和真菌均具有抑制活性.

Methods of lyses by amino chloride, supersonic destruction of ceils, ion exchange chromatography and SDS- PAGE were used in extraction and analysis of deer neutrophil peptides. After the antimicrobial activities being tested, the minimal inhibition concentrations （MIC） of the extracts were tested by means of micro-titer-plate assay. The results show that there are natural antimicrobial substances in deer leukocyte granules, and the main components are peptides. The MIC tests showed inhibitive activities against Gram-positive and Gram-negative bacteria,and fungi.