高效液相色谱法测定精制冠心片中芍药苷的含量

Content Detemination of Paeoniflorin in Refined Coronary Tablets by HPLC

目的:建立以高效液相色谱法测定精制冠心片中芍药苷含量的方法。方法:色谱柱为Kromasil C18,流动相为乙腈-0.2%磷酸溶液(13∶87),检测波长为230nm,流速为1.0ml/min,柱温为室温,进样量为10μl。结果:芍药苷进样量在0.317μg^1.587μg范围内与峰面积积分值线性关系良好(r=0.9 999),平均回收率为100.1%(RSD=1.46%)。结论:本方法简便、快捷、专属性强、重现性好,可用于精制冠心片的质量控制。

OBJECTIVE： To establish a HPLC method for content determination of paeoniflorin in refined coronary tablets.METHODS：HPLC was performed on Kromasil C18 column at room temperature, the mobile phase consisted of acetonitrile-0.2%phosphoric acid solution（13 ： 87）, the detection wavelength was 230nm with flow rate lml/ min and sample size 10μl. RESULTS： Paeoniflorin was linear with the peak area in the range of 0.317μg～1. 587μg（ r=0.9 999）, the average recovery was 100.1% （RSD=1.46% ） .CONCLUSION： The established method is simple, reliable, reproducible and can be used for the quality control of refined coronary tablets.