极低频三角形电磁场对成骨细胞的影响

The Effects of Extremely Low Frequency Triangular Electromagnetic Fields on Rat Osteoblast

目的:观察三角形极低频电磁场对新生大鼠颅盖骨成骨细胞增殖与分化的影响。方法:采用频率为15Hz、不同强度、不同形状的三角形电磁场作用于成骨细胞,测量成骨细胞的增殖与碱性磷酸酶活性(ALP)。结果:频率为15Hz,强度为4mT～5mT,三角形电磁场能够显著提高成骨细胞的增殖率和ALP的活性,而强度为8mT时则显著抑制成骨细胞的增殖与ALP的活性;不同形状的三角形电磁场也明显影响成骨细胞的功能,其中以ρ为38%、85%对成骨细胞的影响最为明显。结论:电磁场的生物学效应依赖于其参数特征。

Objective： Rat fetal carlarial osteoblast were used to test the effects of triangular electromagnetic ,field on proliferation and differentiation. Methods： We used different amplitude and form of 15 Hz triangular electromagnetic on rat fetal carlarial osteoblast and tested the proliferation and alkaline phosphatase activity of osteoblast. Results： It was found that magnetic field which amplitude range from 4 mT to 5 mT at 15 Hz can significantly promote the proliferation and ALP, but amplitude up to 8 mT dependently decrease the proliferation and ALP of the cells. It was also found that osteoblast were significantly effected by different form of triangular electromagnetic field,especially ρ up to 38% and 85%. Conclusion： The proliferation and differentiation of osteoblast can be affected by triangular electromagnetic field which was characterized by its amplitude,frequency and form.