γ射线照射对单采血小板保存中趋化性细胞因子含量的影响

Effect of Prestorage Gamma Ray Radiation on the Chemokine Levels in Stored Apheresis Platelets

目的:研究γ射线照射对单采血小板保存过程中趋化性细胞因子含量的影响。方法:应用COBE SpectraTM细胞分离机单采血小板,然后以27Gy的γ射线照射,于标准条件下保存5d,间隔取样用ELISA方法检测MCP-3,MCP-1,MIP-1α及RANTES含量。结果:对照组单采血小板在保存1d后,所有趋化性细胞因子含量与保存前比较显著升高(P<0.05),照射组保存3d后,RANTES、MCP-3、MIP-1α含量与保存前比较无明显差异(P>0.05),但在保存5d后,所有趋化性细胞因子均与保存前比较有明显升高(P<0.05)。在保存1d后照射组所有趋化性细胞因子含量均明显低于对照组(P<0.05)。结论:27G yγ射线照射能抑制单采血小板保存过程中趋化性细胞因子含量的升高,从而可以有效地预防单采血小板输注后的非溶血性发热反应。

Objective： To study the effect of prestorage gamman radiation on the chemokine levels in storage apheresis platelets. Methods： Apheresis platelets were prepared by the COBE spectraTM cell separator,then irradiated by gammaray at the does of 27 Gy stored at standard condition for 5 days.chemokine levels were measured by ELISA at various storage time. Results： The contents of all chemokine in apheresis platelets of control group after 1 days storage were significantly different from those of prestorage （P〈 0,05）.RANTES,MCP-3 ,MIP-1α in apheresis platelets of irradiated group after 3 days storage compared to those of prestorage showed no significant change （P 〉 0.05）, but after 5 days storage, the content of all chemokines were much higher than those of prestorage（P〈 0.05）.After 1 day storage the contents of all chem okines in radiation group were lower than that of control group （P〈 0.05）. Conclusions： Gamma radiation at 27 Gy is effective in preventing the generation of chemokines in the storage of apheresis platelets.Thus it could prevent monhemolytic febrile transfusion reaction after platelet concentrates transfusion.