SPIO分子探针标记裸鼠肺腺癌移植瘤的磁共振成像及病理学初步研究

In vivo MR imaging and pathology study in mice model labeled with SPIO

目的建立应用超顺磁性氧化铁(SPIO)分子探针氨基硅烷Fe3O4纳米颗粒标记人肺腺癌的裸鼠移植瘤模型,行磁共振成像和病理观察,探讨其临床价值。方法制备氨基硅烷Fe3O4纳米颗粒,分别将标记和未标记肺腺癌细胞株植入裸鼠皮下,观察其成瘤情况,行MR扫描和病理观察。结果标记及未标记纳米分子探针SPIO的肿瘤模型均成功建立,其中前者肿瘤较后者在MR成像中信号强度有明显变化,在T1WI、T2WI、FGR/20°和FGR/70°四个序列中,以FGR/20°变化最明显。病理切片普鲁士兰染色可见肿瘤细胞及坏死组织内有铁染色。结论氨基硅烷Fe3O4纳米颗粒标记人肺腺癌细胞建立裸鼠移植瘤模型稳定可靠,应用磁共振可以对其进行活体监测,提示在临床上具有良好的潜在性应用前景。

Objective To establish mice model bearing transplanted human lung adenocarcinoma labeled with ultrasmall su perparamagnetic iron oxide （SPIO）. and to obtain the MR imaging and pathology results. Methods Aminosilane-coated magnetic particles were produced. Both human lung adenocarcinoma cell line labeling with SPIO or not were planted under the skin of mice, respectively. The development of the tumor was observed, then the MR imaging and pathology results were obtained. Results All the experimental mice have been made transplanted tumor models labeled with SPIO or not. The signal intensity decrease for the labeled tumors with SPIO compared with that for unlabeled tumors was demonstrated on TIWI, T2WI, and FGR/20° and FGR/70° sequences. The percentage change in FGR/20° sequence was the largest. The iron intracellular or in the necrosis could be observed clearly with Prussian blue stain. Conclusion The mice models bearing transplanted human lung adenocarcinoma labeling with aminosilane-coated nano Fe3O4 particles are stable, and they can he observed with 1.5T MR equipment in vivo.